纳米孔16S全长和ITS测序用于在腹腔内感染中识别微生物群
Jian-Jhou Liao1,2, Yong-Sian Chen1, Hui-Chen Lin1
1Division of General Surgery, Department of Surgery, Taichung Veterans General Hospital, Taichung 40705, Taiwan.
Diagnostics (Basel, Switzerland)
|September 13, 2025
概括
牛津纳米孔技术 (ONT) 测序为腹内感染 (IAI) 提供快速和全面的病原体检测. 这种先进的方法可以识别各种微生物,包括厌氧生物和真菌,这些微生物往往被传统文化遗漏,改善了诊断和治疗.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 腹腔内感染 (IAI) 带来了重大的临床挑战,如果不及时治疗,可能会导致严重的后果.
- 传统的基于培养的方法用于IAI的病原体鉴定具有局限性,包括长的周转时间 (TATs) 和难以检测多微生物或无氧感染.
研究的目的:
- 评估牛津纳米孔技术 (ONT) 测序对IAI患者快速和全面的微生物群分析的实用性.
- 将ONT测序与传统的基于培养的方法进行IAI病原体检测的比较.
主要方法:
- 在台中退伍军人总医院对16名IAI患者进行微生物群分析的ONT测序的实施.
- 提取DNA,然后进行全长16SrRNA和ITS区域放大和患者样本的ONT测序.
主要成果:
- 在IAI中,ONT测序确定了各种各样的细菌和真菌,读数的数量差异很大.
- 在下部胃肠道感染中,多微生物和无氧生物丰富的社区普遍存在,而上部胃肠道穿孔显示出不同的微生物特征.
- 测序结果与常见病原体如*Escherichia coli*和*Candida albicans*的培养发现一致,并独特识别了无氧或低丰度的种群.
结论:
- 与传统方法相比,ONT测序在24小时内提供了结果,大大减少了TAT.
- 该技术在培养负病例中成功检测到病原体,突出显示了其在复杂的AIIs中的优势.
- ONT测序是快速和全面诊断IAI的一个有价值的工具,改善了患者管理.
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