坎佛树中的AP2/ERF基因家族:结构,进化和转录对虫感染的反应
Jiexi Hou1,2, Jinrui He2, Yiran Liu2
1Jiangxi Provincial Engineering Research Center for Seed-Breeding and Utilization of Camphor Trees, Nanchang 330099, China.
Plants (Basel, Switzerland)
|September 13, 2025
概括
这项研究对坎佛树中154个AP2/ERF基因进行了鉴定,揭示了它们在生长,应激反应和防御方面的作用. 基因复制和基因表达分析提供了对树适应性和弹性的见解.
科学领域:
- 植物基因组学 植物基因组学
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- AP2/ERF转录因子家族对植物发育和应激适应至关重要.
- 在树 (Camphora officinarum) 中,AP2/ERF基因的基因组多样性和功能作用在很大程度上是未知的.
研究的目的:
- 在树基因组中识别和描述AP2/ERF基因家族.
- 研究推动AP2/ERF家族扩张的进化机制.
- 分析AP2/ERF基因在茎发育过程中的表达模式以及对病原体感染的反应.
主要方法:
- 对AP2/ERF基因进行全基因组识别.
- 基因家族扩张的合成和结构分析.
- 对于cis-regulatory元素的推动者分析.
- 在发育和生物压力期间使用RNA测序进行基因表达概况.
主要成果:
- 确定了154个AP2/ERF基因,其中ERF亚家族占主导地位.
- 同步和细分重复被确定为基因家族扩张的主要驱动因素.
- 结构分析揭示了AP2和ERF/RAV亚家族的独特进化路径.
- 促进体分析表明它们在激素信号和应激反应中的作用.
- 表达式分析显示了构成性和特定阶段的表达模式,在Epicoccum poaceicola感染下有差异性调节.
结论:
- 这项研究破译了树AP2/ERF基因的基因组架构和结构多样性.
- 表达力学突出了它们在树发育和适应生物压力的作用.
- 这些发现为通过分子育种改善木质植物的抗压能力提供了基础.
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