通过递归OPLSDA建模对米特拉基纳物种的叶片代谢的跨种类和环境影响

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Tushar Andriyas¹, Nisa Leksungnoen^1,2,3, Suwimon Uthairatsamee¹

¹Department of Forest Biology, Faculty of Forestry, Kasetsart University, Bangkok 10900, Thailand.

Plants (Basel, Switzerland)

|September 13, 2025

概括

这项研究使用了分层的OPLS-DA来分析植物代谢组,区分了四种Mitragyna物种. 它确定了关键的代谢物,并突出了对化学资料的环境影响.

科学领域:

植物代谢学植物代谢学
化学分类学化学分类学
环境对植物化学的影响.

背景情况:

二次代谢物概况对于了解植物物种及其环境至关重要.
区分复杂的植物代谢需要先进的分析方法.

研究的目的:

用一种新的分层OPLS-DA方法对四种泰国米特拉基纳物种的叶子代谢组进行分类.
为了确定关键的二次代谢物,负责跨物种和环境的区别.
在多类代谢分析中评估递归二进制分类的有效性.

主要方法:

气色谱-质谱 (GC-MS) 用于代谢物注释.
层次直角投射到潜结构差异分析 (OPLS-DA) 进行分类.
递归二进制分类来确定跨物种和环境影响.

主要成果:

与M.diversifolia,M.hirsuta和M.rotundifolia相比,Mitragyna speciosa表现出一个明显的叶子代谢组.
M. hirsuta和M. rotundifolia的代谢体显著重叠,而M. diversifolia则表现出区域代谢变异.
关键的歧视性代谢物包括米特拉基宁,异基林,烯和香草酸.

结论:

层次化的OPLS-DA有效地区分了复杂的植物代谢组,捕捉了跨物种和环境变异.
递归方法提供了生物相关的类结构,而无需手动对对分析.
结果支持使用这种方法用于高维多类植物代谢学.

关键词:

米特拉吉纳物种的种类.集群集成是指集群集成.多类建模的多类建模没有目标的代谢学.

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