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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Experimental RNAi02:15

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Translational Regulation01:29

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Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
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相关实验视频

Updated: Jan 18, 2026

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
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Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

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识别米LncRNAs及其在米爆电阻网络中的作用,使用转录组和转录组.

Xiaoliang Shan1, Shengge Xia1,2, Long Peng3,4

  • 1State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.

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概括
此摘要是机器生成的。

这项研究确定了新的长非编码RNAs (lncRNAs),通过调节激素通路来增强米爆抗性. 这些发现为开发抗病米品种提供了分子基础.

关键词:
马格纳波特花 (Magnaporthe oryzae) 是一个很大的植物.在WGCNA中,WGCNA是WGCNA.ceRNARNA是什么意思激素信号传递的激素信号传递.长长的非编码RNAs.植物免疫力 植物免疫力

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科学领域:

  • 植物分子生物学 植物分子生物学
  • 植物病理学 植物病理学
  • 基因组学和生物信息学

背景情况:

  • 长非编码RNAs (lncRNAs) 在植物免疫力中至关重要,但它们在抗Magnaporthe oryzae (大米爆发) 抗抗米的功能尚不清楚.
  • 准确识别lncRNAs对于理解它们在植物防御机制中的调节作用至关重要.

研究的目的:

  • 开发一个优化的管道来识别米的lncRNAs,并调查它们在抗M. oryzae的米耐药性中的作用.
  • 阐明lncRNAs对大米爆裂耐药性的分子机制,重点关注激素信号通路.

主要方法:

  • 整合翻译组和基因组数据以创建一个优化的蛋白质编码数据集.
  • 开发了"RiceLncRNA"管道,用于使用链特异性RNA测序 (ssRNA-seq) 数据准确识别lncRNA.
  • 采用加权基因共同表达网络分析 (WGCNA) 和竞争性内源性RNA (ceRNA) 网络分析.

主要成果:

  • 鉴定了9003个高保证度的米 lncRNA,显著提高了比传统方法的准确性.
  • 耐药大米品种中的差异表达的lncRNAs (DEL) 与酸 (SA) 和auxin (IAA) 生物合成有关,而敏感品种显示碳代谢中的丰富.
  • 关键的lncRNA可能会作为miRNA海绵,调节类似受体的激酶 (RLKs),耐药性 (R) 蛋白和激素信号通路 (雅斯蒙酸 (JA),乙烯 (ET),IAA).

结论:

  • 这项研究提出了一个优化的米lncRNA注释框架,并揭示了在米爆发抵抗中激素信号通路的lncRNA介导调节.
  • 这些发现为培育抗病米品种提供了关键的分子洞察力.