2'F-RNA对I型结构和稳定性的影响
Cristina Ugedo1, Arnau Domínguez2, Irene Gómez-Pinto1
1Instituto de Química Física Blas Cabrera (IQF), CSIC, Serrano 119, 28006 Madrid, Spain.
Molecules (Basel, Switzerland)
|September 13, 2025
概括
将2′-- рибоцитидин纳入DNA的i-动机可以使结构不稳定或稳定,这取决于替代水平. 部分替代在酸性pH下支持稳定的i-motif形成,而作为一种有用的光谱探针.
科学领域:
- 核酸化学的核酸化学
- 结构生物学 结构生物学
- 生物物理化学 生物物理化学
背景情况:
- I-图案是依赖pH的DNA结构,由C•C+基对稳定.
- 化学修饰可以改变i-motif的稳定性,但糖的修饰往往会破坏结构.
研究的目的:
- 为了研究2 - - рибоцитидин (2F-riboC) 纳入i型形成DNA序列的结构和热力学效应.
- 探索2F-riboC作为i-motif动态的光谱探测器的实用性.
主要方法:
- 紫外线光谱法 紫外线光谱法
- 质子核磁共振 (1H NMR) 光谱学.
- -19核磁共振 (19F NMR) 光谱学
- 高分辨率的NMR结构确定.
主要成果:
- 完全用2F-riboC破坏稳定的i-motifs进行替换.
- 在酸性pH (pH5) 时,部分替代 (1-2个链) 产生了稳定的i基因结构.
- 核磁共振结构显示了明确的i-动图,保留了C•C+配对和特有的NOE.
- 细胞酸糖采用北向,将导向小槽.
- 19F核磁共振证实了折叠和展开状态之间的缓慢交换,使同时检测成为可能.
结论:
- 2 F-riboC的加入以取代依赖的方式调节i-motif的稳定性.
- 部分2F-riboC替代支持在酸性pH下形成i-motif.
- 在2糖位置的作为核酸研究的有效光谱探针,特别是用于i动因动态.
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