Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jan 18, 2026

Microfluidic Chip Fabrication and Method to Detect Influenza
09:43

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

15.5K

具有垂直结构的高密度微流体芯片用于数字PCR

Peng Sun1, Huaqing Si2, Gangwei Xu3

  • 1School of Information Technology, Luoyang Normal University, Luoyang 471934, China.

Sensors (Basel, Switzerland)
|September 13, 2025
PubMed
概括
此摘要是机器生成的。

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

MonSter++: Unified Stereo Matching, Multi-View Stereo, and Real-Time Stereo With Monodepth Priors.

IEEE transactions on pattern analysis and machine intelligence·2026
Same author

Binary spatial-spectral encoding and decoding for ultra-multiplexed digital PCR: Breaking the fluorescence channel limit.

Biosensors & bioelectronics·2026
Same author

Self-Powered Microfluidic System Based on Double-Layer Rotational Triboelectric Nanogenerator.

Micromachines·2025
Same author

A Cost-Effective Reference-Less Semiconductor Ion Sensor with Anodic Aluminum Oxide Film.

Sensors (Basel, Switzerland)·2025
Same author

IGEV++: Iterative Multi-Range Geometry Encoding Volumes for Stereo Matching.

IEEE transactions on pattern analysis and machine intelligence·2025
Same author

Chip-based digital PCR with large-field one-shot imaging for high-sensitivity nucleic acid quantification.

Biosensors & bioelectronics·2025
Same journal

RETRACTED: Zhang et al. A Novel Framework for Reconstruction and Imaging of Target Scattering Centers via Wide-Angle Incidence in Radar Networks. <i>Sensors</i> 2025, <i>25</i>, 6802.

Sensors (Basel, Switzerland)·2026
Same journal

Enhancing Unsupervised Multi-Source Domain Adaptation for Person Re-Identification via Mixture of Experts and Graph-Based Relation.

Sensors (Basel, Switzerland)·2026
Same journal

Development of an Instrumented Glove for Palmar Pressure Assessment in Kayakers.

Sensors (Basel, Switzerland)·2026
Same journal

Development and Experimental Validation of an Autonomous IoT-Based Monitoring System for Real-Time Water Quality Assessment in the Amazon River.

Sensors (Basel, Switzerland)·2026
Same journal

Semi-Supervised Adversarial Learning Framework for Controller Area Network Bus Intrusion Detection.

Sensors (Basel, Switzerland)·2026
Same journal

Smart Optimization Method for Safety Signs in Innovative Manufacturing Environments Integrating Industrial Field IoT Sensors and Knowledge Graphs.

Sensors (Basel, Switzerland)·2026
查看所有相关文章

我们开发了一种用于数字PCR (聚合酶连锁反应) 的新型微流体芯片,可以提高灵敏度和动态范围. 这种低成本的垂直结构芯片可以改善核酸量化,用于癌症查等应用.

科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 微流体学 微流体学

背景情况:

  • 数字PCR (聚合酶连锁反应) 对于癌症查等领域的绝对核酸量化至关重要.
  • 目前的数字PCR方法面临的局限性包括高成本,复杂的程序和有限的检测动态范围.

研究的目的:

  • 为数字PCR开发一个具有成本效益和高性能的微流体芯片.
  • 通过创新的垂直结构,克服现有的数字PCR技术的局限性.

主要方法:

  • 一个新的三层玻璃-PDMS-玻璃微流体芯片被设计成一个高密度的垂直结构.
  • 该芯片在聚二甲基酸 (PDMS) 层内包含3万个反应室 (每一个为0.713nL).
  • 垂直设计将室数和总体体积增加50%,而不会改变芯片足迹或室尺寸.

主要成果:

  • 在理论上,垂直结构的芯片可以实现核酸检测的动态范围接近105.
  • 用连续稀释的KRAS等离子体DNA进行的实验证实了芯片在定量检测中的准确性和可靠性.
  • 与传统设计相比,芯片表现出增强的灵敏度和显著更大的检测动态范围.

结论:

关键词:
检测准确度 检测准确度 检测准确度数字PCR是数字的PCR.动态范围的动态范围.这是一个微流体芯片.垂直结构垂直结构垂直结构

更多相关视频

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

21.7K
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.5K

相关实验视频

Last Updated: Jan 18, 2026

Microfluidic Chip Fabrication and Method to Detect Influenza
09:43

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

15.5K
Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

21.7K
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.5K
  • 开发的垂直结构数字PCR芯片提供了简单的制造过程,统一的样本分区和广泛的动态范围.
  • 这一创新有可能降低成本和复杂性,促进生物医学应用中更广泛地采用数字PCR.