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作为乙烯生物合成的潜在抑制剂的aptamers:识别和在的选择

Diana Laura Aparicio-Breceda¹, Cristian Patricia Cabrales-Arellano², Efren Delgado³

¹Department of Chemical and Biochemical Engineering, National Technological Institute of Mexico (TecNM)-Durango Institute of Technology (ITD), Blvd. Felipe Pescador 1830, Nueva Vizcaya, Durango 34080, Durango, Mexico.

International journal of molecular sciences

|September 13, 2025

概括

研究人员通过计算识别了针对乙烯生物合成中的关键酶的新型体. 这些体显示出延迟水果成熟和减少食物浪费的潜力,为收获后损失减轻提供了一个有希望的途径.

科学领域:

生物技术是生物技术.
计算生物学计算生物学
农业科学农业科学

背景情况:

食物浪费是一个重大的全球问题,在2020年,全球有13.3%的食物被浪费.
乙烯生物合成对于水果成熟,植物生长和衰老至关重要.
传统的体选择是昂贵的,低效的,耗时的.

研究的目的:

使用in silico方法识别具有高预测亲和力对ACC合成酶和ACC氧化酶的aptamer.
建立一个计算基础,用于开发基于aptamer的抑制剂来控制水果成熟.
通过针对性干预乙烯生物合成来减少收获后的食物损失.

主要方法:

使用in silico分子对接来评估aptamer-enzyme的结合潜力.
采用ZDOCK用于初步查阿巴胺-酶相互作用.
使用HDOCK进行了细致的结合亲和力和特异性的分析.

主要成果:

胺基AB451和ABR6P.1对ACC合成酶表现出有希望的结合亲和力.
胺基RO33828和O0O6O1显示了ACC氧化酶的最佳预测结合.
这些发现为新型体开发提供了计算基础.

结论:

针对乙烯生物合成酶的阿普坦的计算识别是可行的和高效的.
已识别的体具有作为抑制剂延迟水果成熟的潜力.
需要进一步的实验验证,以确认其抑制功能和有效性,以减少收获后损失.

关键词:

在ACC氧化酶中,它们是ACC合成酶.它们是RNA吸收酶.分子对接的分子对接.

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