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甲索特雷克萨和托法西提尼布对线粒体功能和氧化应激在人体突细胞的作用In Vitro

Valentina Mihaylova^1,2, Desislav Tomov², Rositsa Karalilova^3,4

¹Department of Medical Biology, Medical University, Blvd. Vasil Aprilov 15A, 4000 Plovdiv, Bulgaria.

International journal of molecular sciences

|September 13, 2025

概括

甲三甲酸恶化了线粒体功能,并增加了类风湿性关节炎 (RA) 结膜细胞中的氧化应激. 然而,托法西替尼在这种体外RA模型中改善了线粒体参数.

科学领域:

线粒体生物学线粒体生物学
免疫学免疫学免疫学
药理学药理学是指药理学的学科.

背景情况:

类风湿性关节炎 (RA) 是一种自身免疫性疾病,会影响突.
线粒体功能障碍是RA发病的关键因素.
了解药物对细胞代谢的影响对于RA治疗至关重要.

研究的目的:

调查甲状腺素和托法西提尼布对线粒体功能和氧化应激的影响.
用SW982突细胞系在风湿性关节炎的体外模型中评估这些影响.
在线粒体健康方面,比较甲状腺与托法西替尼的体外疗效.

主要方法:

利用SW982突细胞系,用TNF-alpha刺激,作为风湿性关节炎的体外模型.
给细胞培养物注射甲和托法西提尼布.
使用代谢测试评估线粒体功能.
通过色谱分析量化氧化应激,特别是测量8-ISOPGF2A水平.

主要成果:

托法西替尼治疗导致ATP水平增加 (p < 0.0001) 和减少质子泄漏 (p < 0.0003).
甲托雷克萨特治疗导致ATP产生减少 (p < 0.0096) 和增加质子泄漏 (p < 0.0001).
观察到8-ISOPGF2A的两倍增加,表明氧化应激升高.

结论:

在RA细胞模型中,甲托雷克萨特在体外诱导线粒体功能障碍和氧化应激.
托法西提尼布显示线粒体参数的部分改善.
这些发现突出了RA治疗药物对细胞生物能学和氧化平衡的差异性影响.

关键词:

这是一个ISOPGF2A标准.SW98282 在线观看线粒体功能线粒体功能氧化应激是一种氧化应激.

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