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在体中识别circPIM1/miR-16-5p/miR-195-5p/PIM1反复复发的2级脑膜瘤中的前循环

Giuseppe Sotera¹, Carla Forte², Daniele Giuseppe D'Urso^3,4

¹Department of Medical, Surgical Sciences and Advanced Technologies "G.F. Ingrassia", Neurological Surgery, Policlinico Rodolico-San Marco University Hospital, University of Catania, Via Santa Sofia, 87, 95123 Catania, Italy.

International journal of molecular sciences

|September 13, 2025

概括

一个新的具有竞争力的内源性RNA (ceRNA) 网络,涉及PIM1,circRNAs 0076215/0076216和miRNAs 16-5p/195-5p,可能会导致脑膜瘤 (MNG) 复发. 这项研究确定了PIM1作为侵略性MNG中的潜在瘤基因.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

一个34个转录生物标志物 (34HR-MNG) 预测脑膜瘤 (MNG) 瘤的结果,包括复发.
了解34HR-MNG转录调节背后的分子机制对于MNG复发预测至关重要.

研究的目的:

构建一个具有竞争力的内源RNA (ceRNA) 网络,以阐明调节34HR-MNG转录的分子机制.
确定该网络在MNG复发中的功能作用.

主要方法:

使用MiRTarbase和ENCORI数据库构建一个ceRNA网络.
对circRNA宿主基因表达与MNG临床/分子特征的相关性分析 (RNA-seq数据集GSE189672).
通过qRT-PCR验证circRNA和宿主基因表达.

主要成果:

在世卫组织2级与1级和复发与非复发的世卫组织2级MNG中,Pim-1原基因,氨酸/氨酸激酶 (PIM1) 被上调.
在WHO2级MNG中,PIM1表达与Ki-67和NF2相关.
循环RNAs 0076215和0076216,来自PIM1,海绵瘤抑制miRNAs (16-5p,195-5p),导致PIM1上调.

结论:

皮姆1作为一种瘤基因,与世卫组织2级MNG复发有关.
已识别的ceRNA网络,包括PIM1,circRNAs 0076215/0076216和miRNAs 16-5p/195-5p,通过feed-forward循环驱动PIM1上调,从而导致MNG复发.

关键词:

具有竞争力的内源性RNA (ceRNA) 网络.进食前传基因表达循环脑膜瘤是指脑膜的一个部分.复发性复发性复发性

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