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基于结构的工程 PTPsigma Ectodomain 的增强可溶性和生产力

Sung Ho Park1, Woochan Lim1, Jian Kang1

  • 1Department of Bioengineering, College of Engineering, Hanyang University, Seoul 04673, Republic of Korea.

International journal of molecular sciences
|September 13, 2025
PubMed
概括

工程化蛋白氨酸酸酶受体sigma (PTPRS) 类似Ig的域显示出更好的溶解性和表达产量. 这种蛋白质工程增强了PTPRS调制的治疗潜力.

关键词:
生产收益率生产收益率蛋白质氨酸酸酶受体sigma 是一个蛋白质氨酸酸酶受体.溶解度 溶解度 溶解度 溶解度基于结构的工程.治疗应用 治疗应用

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Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
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