肝组织病理学益处,微生物成本:口服万科米可以缓解非酒精性脂肪性肝病,同时破坏菌群
Gül Çirkin1, Selma Aydemir2, Burcu Açıkgöz3
1Department of Pediatric Gastroenterology Hepatology and Nutrition, Faculty of Medicine, Dokuz Eylul University, Izmir 35340, Türkiye.
在非酒精性脂肪肝炎 (NASH) 鼠标模型中,口服万科米辛改善了肝损伤,但改变了肠道微生物群. 这项研究强调了NASH疗法的肝脏益处和微生物组破坏之间的权衡.
科学领域:
- 肝病学 肝病学是一种肝病学.
- 微生物组研究的研究.
- 胃肠病学 胃肠病学
背景情况:
- 非酒精性脂肪性肝病 (NAFLD) 和非酒精性脂肪性肝炎 (NASH) 涉及肠-肝轴相互作用.
- 人们越来越认识到肠道微生物群在NASH病变发生中的作用.
- 准肠道微生物群是NASH的潜在治疗策略.
研究的目的:
- 为了评估口服万科米辛对肝损伤和内微生物群的作用,在一种甲素胆缺乏 (MCD) 饮食诱导的NASH小鼠模型中.
- 评估肝脏中的组织学变化,细胞因子水平和免疫组织化学标志物.
- 分析肠道微生物组成和多样性的变化.
主要方法:
- 雄性C57BL/6J小鼠被食标准 (STD) 或MCD饮食,含或不含万科米辛 (VANC),持续10周.
- 肝脏组织被分析为组织学,细胞因子 (IL-6,IL-8,TGF-β1) 和标记物 (ubiquitin,CK18).
- 使用16S rRNA测序来评估Cecal微生物群的组成.
主要成果:
- 这种MCD饮食诱导了NASH的特征,包括巨型肌肉肥胖症和叶片炎症.
- 范科米辛治疗将肥胖症转移到微风脉状模式,并降低了肝细胞损伤标志物 (CK18,ubiquitin).
- 范科米辛显著改变了肠道微生物群,减少了多样性,并耗尽了像乳杆菌这样的有益细菌,同时丰富了病原生物.
结论:
- 在MCD饮食诱导的NASH模型中,口服万科米辛改善了肝损伤.
- 范科米辛彻底重塑肠道微生物群,导致一种低多样性,富含病原生物的生态系统.
- 这些发现表明一种治疗性权衡:肝脏益处与微生物组破坏,指导未来针对微生物群的NASH策略.
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