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在二维和三维三阴性乳腺癌模型中准EGFR/IGF-IR功能交叉,以评估瘤进展

Spyros Kremmydas¹, Chrisavgi Gourdoupi¹, Zoi Piperigkou¹

¹Biochemistry, Biochemical Analysis & Matrix Pathobiology Research Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26504 Patras, Greece.

International journal of molecular sciences

|September 13, 2025

概括

三阴性乳腺癌 (TNBC) 研究表明,抑制表皮生长因子受体 (EGFR) 和类似胰岛素的生长因子I受体 (IGF-IR) 影响细胞迁移和入侵. 三维 (3D) 模型揭示了对治疗耐药性的关键见解.

科学领域:

在瘤学瘤学.
细胞生物学细胞生物学
生物化学生物化学

背景情况:

三阴性乳腺癌 (TNBC) 是一种具有不良预后的侵袭性亚型.
包括EGFR和IGF-IR在内的受体氨酸激酶 (RTK) 与乳腺癌进展和治疗耐药性有关.
需要更准确的临床前模型来反映瘤微环境 (TME).

研究的目的:

通过2D和3D培养模型研究EGFR和IGF-IR在TNBC进展中的作用.
评估结合EGFR和IGF-IR抑制在TNBC中的疗效.
为了比较2D与3D模型在评估治疗反应中的实用性.

主要方法:

利用TNBC细胞系的二维 (2D) 和三维 (3D) 球形培养模型.
评估了EGFR和IGF-IR的单独和联合抑制的影响.
分析了细胞功能,包括迁移,上皮细胞转化为介质酶 (EMT) 标记,矩阵金属蛋白酶 (MMP) 和球状细胞生长/扩散.

主要成果:

EGFR和IGF-IR都是TNBC细胞迁移,EMT标志物表达和MMP活性的关键调节者.
3D球状体模型为治疗反应提供了额外的见解,包括球状体的生长和传播.
在3D模型中,EGFR和IGF-IR的联合抑制表明了对TNBC进展的治疗依赖性影响.

结论:

在TNBC的侵略性和转移中,EGFR和IGF-IR起着重要的作用.
三维 (3D) 球形模型优于二维培养,用于评估针对TNBC的治疗策略.
对EGFR和IGF-IR的双抑制值得进一步研究,作为TNBC的潜在治疗方法.

关键词:

2D与3D细胞培养的区别 3D球形形状的球形体.欧洲农业基金会 (EGFR) 是一个基金.国际海事基金-国际海事基金 (IGF-IR) 是一个国际海事基金.乳腺癌乳腺癌乳腺癌

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