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Updated: Jul 18, 2026

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

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通过UHPLC-QqQ MS进行RNA修饰分析的协议

Yueh-Lin Tsai1, Nan Dai1, Ivan R Corrêa1

  • 1Research Department, New England Biolabs, Inc., Beverly, MA 01915, USA.

STAR protocols
|September 13, 2025
PubMed
概括

这项研究介绍了一种使用超高性能液态染色学和质谱学 (UHPLC-QqQ MS) 识别和量化RNA修饰的协议. 这种方法可以在古老的,细菌的和真核细胞中进行分析.

关键词:
生物技术和生物工程化学家 化学家 化学家质谱测量质量谱测量

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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...