Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A DNA-guided prokaryotic argonaute enables programmable RNA cleavage for sequencing and quality control of <i>in vitro</i>-transcribed RNAs.

Molecular therapy. Nucleic acids·2026
Same author

Knockout of OsRbohD (the NADPH Oxidase Gene) Enhances Saline-Alkaline Stress Tolerance and Grain Yield in Rice by Reducing ROS Accumulation.

Rice (New York, N.Y.)·2026
Same author

Semirandom DNA adducts regulate a filamentous defense-associated reverse transcriptase.

Nature structural & molecular biology·2026
Same author

Regulation of exosomal miR-513a and miR-149 expression by APE1 in PD-L1 and TCR immune regulation pathways to promote immune evasion in osteosarcoma.

Cancer genetics·2026
Same author

Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducens.

Nucleic acids research·2026
Same author

Innovative use of a long transparent cap for closure of large duodenal defects post endoscopic full-thickness resection: a case report.

iGIE : innovation, investigation and insights·2026

相关实验视频

Updated: Apr 2, 2026

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

8.7K

通过UHPLC-QqQ MS进行RNA修饰分析的协议.

Yueh-Lin Tsai1, Nan Dai1, Ivan R Corrêa1

  • 1Research Department, New England Biolabs, Inc., Beverly, MA 01915, USA.

STAR protocols
|September 13, 2025
PubMed
概括
此摘要是机器生成的。

这项研究介绍了一种使用超高性能液态染色学和质谱学 (UHPLC-QqQ MS) 识别和量化RNA修饰的协议. 这种方法可以在古老的,细菌的和真核细胞中进行分析.

关键词:
生物技术和生物工程化学家 化学家 化学家质谱测量质量谱测量

更多相关视频

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

2.4K
Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.9K

相关实验视频

Last Updated: Apr 2, 2026

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

8.7K
2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

2.4K
Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.9K

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 分析化学 分析化学

背景情况:

  • 已知有170多种不同的RNA修饰,在细胞过程中起着至关重要的作用.
  • 准确识别和量化这些修饰对于理解RNA功能至关重要.

研究的目的:

  • 提出一个详细的协议,用于识别和量化RNA中修饰的核化物.
  • 建立一个可靠的方法,适用于不同的生活领域.

主要方法:

  • 使用超高性能液态染色学与三重四极质谱学 (UHPLC-QqQ MS) 相结合.
  • 涉及准备核酸标准,确定保留时间和质量转换,并构建校准曲线.
  • 包括RNA消化和样本分析程序.

主要成果:

  • 该协议已成功应用于分析古代,细菌和真核细胞中的RNA修饰.
  • 证明了UHPLC-QqQ MS对于精确核酸量化的能力.

结论:

  • 开发的协议为全面的RNA修饰分析提供了强大的方法.
  • 促进对不同生物体RNA修饰的生物学意义的进一步研究.