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定义Brucella melitensis的流行病学值:一个欧洲多中心研究

Flavia Dematheis¹, Joseph Papaparaskevas², Erika Matuschek³

¹Bundeswehr Institute of Microbiology, Central Diagnostic Department, Munich, Germany.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases

|September 13, 2025

概括

标准化抗微生物敏感性测试 (AST) 方法对Brucella melitensis进行了验证. 建立了流行病学截止值 (ECOFF),有助于检测对关键抗生素的潜在耐药机制.

科学领域:

微生物学微生物学
传染性疾病传染性疾病
抗微生物耐药性抗微生物耐药性

背景情况:

布鲁塞洛斯主要是由布鲁塞拉梅利坦尼斯 (Brucella melitensis) 引起的,具有严重的慢性感染和复发风险.
标准化抗微生物敏感性测试 (AST) 程序和临床断点对于有效的患者管理和治疗结果至关重要.
全欧洲布鲁塞拉参考实验室网络与EUCAST合作,解决了对标准化AST的需求.

研究的目的:

为了建立标准化的AST方法来检测Brucella melitensis.
为了确定野生类型 (WT) 最低抑制度 (MIC) 和区域直径分布.
为九种具有治疗意义的抗微生物药物设定流行病学截止值 (ECOFF).

主要方法:

在六个欧洲研究中心共测试了499种B. melitensis菌株.
微稀释 (BMD) 和磁盘扩散 (DD) 方法用于AST.
根据EUCAST标准操作程序 (SOP) 10.2进行ECOFF确定,MIC和抑制区直径进行了策划.

主要成果:

对使用BMD和DD的测试抗菌剂观察到假设的野生类型分布.
对所有药物确定了MIC ECOFF,其中6个单元显示MICs略高,这表明可能存在对利芬素,菌素和trimethoprim-sulfamethoxazole的抗药性机制.
已经为利芬素和 ceftriaxone 建立了 ECOFF,并为 ciprofloxacin,levofloxone,gentamicin 和 streptomycin 建立了暂定的 ECOFF.

结论:

对B. melitensis的标准化BMD和DD方法得到了验证.
欧卡斯特利用AST结果来设定ECOFF,这些ECOFF被纳入欧卡斯特临床断点表 (v14.0).
这些进展使得B. melitensis中抗菌素耐药性发展的敏感检测和监测成为可能.

关键词:

布鲁塞拉梅利坦尼斯 (Brucella melitensis) 是一种植物.对抗微生物的敏感性测试.临床断点临床断点流行病学界限值是指流行病学界限值.野生类型的MIC分布

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