CotVW复合体的丝状结构是Bacillus subtilis内胞的地蛋白
Eunbyul Jo1, Doyeon Kim1, Yeongjin Baek1
1Research Institute of Agriculture and Life Sciences, Department of Agricultural Biotechnology, CALS, Seoul National University, Seoul, Republic of Korea.
The Journal of biological chemistry
|September 13, 2025
概括
细菌细菌的子皮蛋白CotV和CotW形成了一个独特的螺旋结构. 这种CotVW复合体通过pH-依赖的相互作用保持子表面完整性,这对于细菌的弹性至关重要.
科学领域:
- 微生物学 微生物学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- 细菌细菌内体具有保护性的多层结构,包括最外层的地.
- 蛋白质CotV和CotW对于地形成和子表面完整性至关重要,但它们的功能尚不清楚.
研究的目的:
- 为了确定CotVW复合体在Bacillus subtilis子皮形成中的结构和功能.
主要方法:
- 使用冷电子显微镜 (cryo-EM) 来确定CotVW复合物的结构.
- 结构分析的重点是确定表面特性和潜在的相互作用地点.
主要成果:
- 科特VW综合体呈现出一个丝状螺旋结构.
- CotVW具有负电荷的表面,可以调解pH依赖的静电相互作用,特别是在pH 6.0时与histidine和正电荷的残留物.
结论:
- 这项研究阐明了CotVW功能在子组织中的分子基础.
- 科特维维在维护细菌细菌子表面完整性和弹性方面发挥着关键作用.
相关概念视频
Endospores and Sporulation
5.0K
Endospores are specialized, dormant cells primarily formed by Gram-positive bacteria, including Bacillus and Clostridium, enabling survival under extreme environmental conditions. Due to their unique composition and formation process, these structures are highly resistant to physical and chemical insults, such as extreme heat, ultraviolet and ionizing radiation, desiccation, and toxic chemicals. Rare instances of endospore-like structures have also been observed in some Gram-negative bacteria,...
5.0K
Cytoskeletal Proteins in Bacteria
4.1K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
4.1K
Assembly of Cytoskeletal Filaments
27.1K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
27.1K
Gene Regulation During Sporulation
445
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
445
Bacterial Phylum Actinobacteria
619
Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
619
The Structure of Intermediate Filaments
5.6K
The intermediate filaments are one of three widely studied cytoskeletal filaments. They are so named as their diameter (10 nm) is in between that of microfilaments (7 nm) and the microtubules (25 nm). These filaments are highly stable and can remain intact when exposed to high salt concentrations and detergents. These filaments are responsible for providing stability and mechanical support to the cells. They also help in cell adhesion and maintaining tissue integrity.
Intermediate...
Intermediate...
5.6K


