基于微生物联盟的化合物酶如何影响抗生素耐药性基因的结合性转移途径?
Lihui Cui1, Mengshan Gao1, Jiaxin Chen1
1School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
Bioresource technology
|September 13, 2025
概括
基于微生物联盟的复合酶 (MCE) 预处理通过抑制等离子体结合,显著减少了抗生素耐药性基因 (ARG) 的转移. 这一发现为打击ARG在环境中的传播提供了新的策略.
科学领域:
- 环境微生物学环境微生物学
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 抗生素耐药性基因 (ARG) 构成了严重的环境和公共卫生威胁.
- 基于微生物联盟的化合物酶 (MCE) 显示出在食品废弃物生物转化过程中减少ARG传播的潜力.
- 通过MCE影响ARG转移的精确机制在很大程度上仍未被阐明.
研究的目的:
- 调查MCE预处理对RP4等离子体介导的ARG结合转移的影响.
- 阐明涉及MCE介导ARG转移调节的潜在分子和细胞机制.
- 在食品废弃物模型中验证MCE预处理对ARG转移的抑制作用.
主要方法:
- 对RP4等离子体结合转移频率的分析.
- 评估细胞外聚合物物质 (EPS) 组成.
- 对生物膜形成和细胞间细菌接触的评估.
- 测量ATP合成和细菌运动.
- 结合性转移基因的基因表达分析.
- 在构建的食物浪费模型中进行验证.
主要成果:
- MCE预处理减少了71%的等离子体结合转移频率.
- MCE预处理减少了水友性EPS成分,减少了生物膜的形成,并破坏了细胞间接触.
- 观察到ATP合成和细菌运动性的变化,影响了等离子体复制和转移通道形成.
- 观察到对联转移基因表达的下调.
- 在食品浪费模型中,MCE预处理减少了病原体负载 (例如,Escherichia-Shigella,Rothia).
结论:
- 通过针对多个细胞和分子过程,MCE预处理有效地破坏了ARG结合转移.
- 这项研究揭示了MCE对ARG传播的抑制作用的关键机制.
- 这些发现为开发针对环境ARG传播的新型干预措施提供了理论基础.
相关概念视频
Conjugation
1.9K
Conjugation is a form of horizontal gene transfer that primarily occurs in bacteria and some archaea, promoting genetic diversity and adaptation. Bacteria can acquire resistance genes through conjugative plasmids, allowing them to survive antibiotic treatments that would otherwise be lethal. This process involves direct contact between cells through specialized structures such as the sex pilus and is mediated by conjugative plasmids, including the F (fertility) factor.Conjugation requires...
1.9K
Mechanism of Conjugation
845
Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...
845
Antibiotic Selection
59.4K
Overview
59.4K
Transformation
709
Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...
709
Development of Antibiotic Resistance
1.3K
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
1.3K
Bacterial Transformation
59.4K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
59.4K


