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基乙基甲酸盐在人类牙纤维细胞中具有基因毒性,但不致变异,这是通过Duplex测序评估的

Renke Perduns¹, Kirstin Vach¹, Nadine Schlueter¹

¹Department of Conservative Dentistry, Periodontology and Preventive Dentistry, Hannover Medical School, Carl-Neuberg-Straße 1, Hannover 30625, Germany.

Dental materials : official publication of the Academy of Dental Materials

|September 13, 2025

概括

基乙基甲酸盐 (HEMA) 导致活性氧物种 (ROS) 和基因毒性. 然而,双重测序 (DS) 在人类牙纤维细胞 (HGF) 中没有发现HEMA诱导的突变,这表明恢复期间的DNA修复或亡.

科学领域:

生物医学科学生物医学科学
毒理学毒理学毒理学
遗传学是一个遗传学.

背景情况:

已知基乙基甲酸盐 (HEMA) 诱导不良影响,包括反应性氧物种 (ROS) 生成和基因毒性.
了解HEMA的突变性潜力对于评估其在各种应用中的安全性至关重要.

研究的目的:

通过双重测序 (DS) 来研究HEMA在人类牙纤维细胞 (HGF) 的变异性.
确定HEMA对ROS生成和基因毒性的度依赖作用.

主要方法:

使用Hoechst33342试验,对细胞毒性的HEMA度进行了优化.
使用2,7-二chlorofluorescin二酸盐量化ROS生成.
基因毒性通过彗星试验进行评估,并使用DS分析了HGF细胞中用HEMA (1μM至3.25μM) 治疗的突变性,然后进行恢复期.

主要成果:

在1.5毫米和3.25毫米时,HEMA暴露显著增加了ROS生成.
在1毫米和3.25毫米的HEMA度下观察到基因毒性.
尽管观察到ROS和基因毒性,但DS与对照组相比,在HEMA治疗的HGF细胞中,突变率没有显著增加.

结论:

通过诱导ROS和基因毒性,HEMA表现出突变性特性.
在经过测试的条件下,使用DS检测到的HGF中没有检测到HEMA诱导的突变.
检测到的突变的缺乏可能归因于DNA修复机制或恢复阶段的亡.

关键词:

细胞毒性细胞毒性双重序列测序是指双重序列测序.基因毒性基因毒性基乙基甲基烯酸盐的基乙基甲基烯酸盐.突变性突变性突变性有反应性氧物种的反应性氧物种.

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