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索波拉 (Sophora flavescens) 水性提取物抑制埃梅里亚菌诱导的炎症反应,并调节MAPK通路

Zhe Zheng¹, Yanchun Wang¹, Yunchao Bian²

¹College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.

Poultry science

|September 14, 2025

概括

这项研究发现,S. flavescens水性提取物 (SFAE) 有效地对抗肉中Eimeria tenella诱导的菌病. SFAE治疗减少了肠道损伤,抑制了炎症,并调节了MAPK信号通路,显示出作为抗菌剂的潜力.

科学领域:

兽医寄生虫学兽医寄生虫学
禽类的健康禽类的健康
自然产品化学自然产品化学

背景情况:

由Eimeria tenella引起的菌病导致家禽由于肠道损伤和生长迟缓而造成严重的经济损失.
传统抗菌剂治疗中药物耐药性的增加需要研究替代疗法,例如草本提取物.
(Salvia flavescens) (S. flavescens) 显示出有希望的抗炎和抗寄生素特性,对菌病控制有意义.

研究的目的:

评估S. flavescens水性提取物 (SFAE) 对白羽毛肉的Eimeria tenella感染的抗菌疗效.
研究SFAE对MAPK信号通路在体外MDBK细胞和体外肉模型中的调节作用.
评估SFAE对炎症性细胞因子概况和肠道组织病理学的影响.

主要方法:

在体内研究涉及四组肉:负控,E. tenella感染 (PC),PC + SFAE和PC + diclazuril.
在体外研究中使用了用SFAE治疗的MDBK细胞来评估其对MAPK通路激活 (p38,MEK酸化) 的影响.
使用了组织病理学检查 (HE染色),血清细胞因子分析 (ELISA) 和分子分析 (qPCR,西部斑).

主要成果:

SFAE治疗显著降低了E. tenella诱导的阴病变,血性腹和死亡率,实现了高的抗菌指数.
SFAE使肉体重增加正常化,并保护了肠道小的完整性,与迪克拉祖里尔组相似.
在抑制IL-10的同时,SFAE抑制了MAPK通路激活 (p38,MEK,ERK,JNK,Raf1) 和促炎细胞因子 (IL-1β,IL-6,TNF-α) 的发生.

结论:

在家禽中,S. flavescens水性提取物对Eimeria tenella具有显著的抗菌疗效.
通过抑制MAPK信号通路并减少炎症,SFAE减轻了E. tenella诱导的肠损伤.
SFAE为管理家禽菌病提供了一个可行的天然替代方案,解决了耐药性问题.

关键词:

埃梅里亚天尼拉 (Eimeria tenella) 是一种植物.这就是MAPKK的意思.在S. flavescensens中使用.

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