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相关概念视频

DNA Isolation01:24

DNA Isolation

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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DNA Isolation01:34

DNA Isolation

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DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
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相关实验视频

Updated: Jan 17, 2026

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
06:53

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

Published on: January 5, 2011

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过器准备:一种简单有效的方法,用于高产量,高纯度的等离子体DNA净化.

Yu-Hsuan Cheng1, Yu-Jiu Wu1, Yung-Chun Shih1

  • 1Institute of Biochemistry and Molecular Biology, National Yang Ming Chiao Tung University, Taipei 112, Taiwan.

New biotechnology
|September 14, 2025
PubMed
概括

Filterprep是一种新的混合等离子体DNA净化方法,在40分钟内提供高产量和纯度. 这种具有成本效益的技术简化了分子生物学应用程序的工作流程.

关键词:
生物技术中的应用.具有成本效益的等离子体准备.高产率等离子体DNA提取分子生物学技术分子生物学技术.质粒DNA净化净化方法

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Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
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Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit

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High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology
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High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology

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相关实验视频

Last Updated: Jan 17, 2026

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
06:53

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

Published on: January 5, 2011

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Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
09:24

Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit

Published on: July 29, 2007

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High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology
13:27

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology

Published on: August 8, 2019

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科学领域:

  • 分子生物学分子生物学
  • 生物技术是生物技术.

背景情况:

  • 等离子体DNA净化至关重要,但往往耗费大量资源.
  • 传统方法可能会产生低纯度的DNA;商业套件昂贵且不透明.

研究的目的:

  • 开发一种具有成本效益,透明和高效的等离子体DNA净化方法.
  • 在产量,速度和成本方面改进现有的商业套件.

主要方法:

  • 一种混合方法,将乙醇沉与单个旋转柱清洁相结合.
  • 被命名为过准备方法.

主要成果:

  • 在大约40分钟内,高纯度等离子体DNA的快速恢复.
  • 产量高达商业中期准备套件的5倍.
  • 纯化DNA与转染,蛋白质表达和记者测定相容.

结论:

  • Filterprep为等离子体DNA净化提供了一个透明,具有成本效益和可扩展的替代方案.
  • 适用于高吞吐量和资源有限的实验室.
  • 纯化等离子体DNA在-20°C下稳定12个月.