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相关概念视频

RNA Structure01:19

RNA Structure

7.1K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
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RNA Structure01:23

RNA Structure

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Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
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Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

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Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
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Nucleic Acid Structure01:25

Nucleic Acid Structure

8.4K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
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RNA Interference01:23

RNA Interference

27.8K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.8K
Nucleic Acids02:43

Nucleic Acids

49.7K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
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相关实验视频

Updated: Jan 17, 2026

An Assay for Quantifying Protein-RNA Binding in Bacteria
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An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

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对IMP2二分化和RNA结合的结构见解.

Stephen A Zorc1, Paola Munoz-Tello2, Timothy O'Leary3

  • 1Department of Molecular Medicine, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, Jupiter, FL, USA; The Skaggs Graduate School of Chemical and Biological Science, The Scripps Research Institute, La Jolla, CA, USA.

Journal of structural biology
|September 14, 2025
PubMed
概括
此摘要是机器生成的。

胰岛素样生长因子2的mRNA结合蛋白2 (IGF2BP2) 主要形成二次体,RNA结合改变其结构. 了解IGF2BP2的完整结构是针对癌症和代谢疾病的药物发现的关键.

关键词:
在HDX-MS中使用HDX-MS.在IGF2BP2BP2中使用.在 IMP2 中,MP2 包含了 IMP2.RNA结合蛋白 (RBP) 是一种RNA结合蛋白.萨克斯 (SAXS) 的时间

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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

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相关实验视频

Last Updated: Jan 17, 2026

An Assay for Quantifying Protein-RNA Binding in Bacteria
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Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
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Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

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科学领域:

  • 生物化学 生物化学
  • 结构生物学 结构生物学
  • 分子生物学分子生物学

背景情况:

  • 胰岛素样生长因子2 mRNA结合蛋白2 (IGF2BP2),也称为IMP2,涉及瘤发生和代谢障碍.
  • 之前的结构研究集中在IMP2的单个域上,使得全长蛋白质的结构和行为没有特征.
  • 了解完整的IMP2的结构对于开发有针对性的药物发现策略至关重要.

研究的目的:

  • 为了研究全长IGF2BP2 (IMP2) 的结构性质和寡合行为.
  • 确定RNA结合对IMP2.2的结构和寡合化的影响.
  • 为针对IMP2.2的潜在治疗干预提供结构性见解.

主要方法:

  • 利用生物物理技术,包括质光测量,-交换与质谱学 (HDX-MS) 相结合.
  • 采用小角度X射线散射 (SAXS) 来建模全长IMP2.2的结构.
  • 研究了RNA结合和不同离子强度对IMP2结构和寡合化的影响.

主要成果:

  • 全长的IMP2存在于多个寡合状态,主要形成二元体.
  • 萨克斯数据建模表明,涉及KH34和RRM1领域的头到尾二维方向.
  • RNA结合会诱导一种伪对称的二维形状,与RNA无状态不同,而寡合化对离子强度敏感.

结论:

  • 本研究提出了全长IMP2.2的第一个结构性特征.
  • 研究结果显示,IMP2的寡合化是动态的,并受到RNA结合和离子条件的调节.
  • 这些见解为在疾病背景下破坏IMP2功能提供了潜在的途径.