由Alcaligenes faecalis使用乳酸作为可再生基质可持续生产的聚乙酸 (PHB) 的表征
Aksha Dhawan1, Dinesh Kalyanasundaram2, Palash Kumar Manna1
1TERI-Deakin Nanobiotechnology Centre, The Energy and Resources Institute, TERI Gram, Gurugram, India.
International journal of biological macromolecules
|September 14, 2025
概括
这项研究优化了使用乳酸作为原料的牛奶酸,一种废物产品,以生产聚乙烯基酸 (PHB). 研究表明,成本效益高,可持续的生物塑料生产具有与商业标准相美的性能.
科学领域:
- 生物技术和材料科学 生物技术和材料科学
- 可持续的高分子.
- 微生物发酵 微生物发酵
背景情况:
- 石化塑料有助于环境污染.
- 可生物降解的塑料,如聚乙酸 (PHB) 提供可持续的替代品.
- 由于加工条件恶劣,利用乳酸等废物流来生产PHB具有挑战性.
研究的目的:
- 以优化使用乳酸作为碳源的聚基酸盐 (PHB) 生产.
- 研究使用*Alcaligenes faecalis*用于从乳酸合成PHB的可行性.
- 描述微生物产生的PHB的特性.
主要方法:
- 使用各种碳和来源,优化了*Alcaligenes faecalis*的发酵条件.
- 使用乳酸和化作为主要基质.
- 通过FTIR,NMR,XRD,DSC和拉伸测试来表征产生的PHB.
主要成果:
- 达到显著的细胞内PHB积累高达88% (w/w) 的细菌细胞干重.
- 微生物产生的PHB表现出与商业PHB相似的特性 (功能组,点,拉伸模量).
- 证实了关键功能组 (CH3和CO) 的存在和相纯度.
结论:
- 乳酸是一种可行和可再生的碳来源,用于经济高效的PHB生产.
- 使用*Alcaligenes faecalis*进行的优化发酵使得PHB产量高.
- 微生物生产的PHB显示了对环保食品包装和生物医学应用的潜力.
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