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在亚洲鱼Clarias batrachus中,Ad4bp/sf-1调节cyp11b1和cyp17a1

Sonika Kar¹, Balasubramanian Senthilkumaran¹

¹Department of Animal Biology, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046 Telangana, India.

General and comparative endocrinology

|September 14, 2025

概括

类固醇原因子1 (SF-1) 和Foxp1调节控制鱼丸功能的关键基因 (cyp11b1,cyp17a1). SF-1对于男性类固醇生成至关重要,影响的产生.

科学领域:

内分泌学在内分泌学.
分子生物学分子生物学
鱼类类固醇生成

背景情况:

鱼的丸功能依赖于类固醇激素.
细胞染色体P450 11B1 (cyp11b1) 和17A1 (cyp17a1) 是关键的类固醇酶.
鱼类中这些酶的调节仍然不太清楚.

研究的目的:

为了研究鱼 (Clarias batrachus) 中cyp11b1和cyp17a1的转录调节.
确定涉及控制这些类固醇基因的关键转录因子.

主要方法:

对鱼 cyp11b1 和 cyp17a1.1 的 5' 上游区域进行克隆和测序.
路西法酶记者在TM3小鼠的莱迪格细胞中进行了测定.
位点定向的突变发生和染色体免疫沉 (ChIP).
在人体内和体外实验中使用人类胆激素 (hCG) 和雄激素进行实验.
小干扰RNA (siRNA) 介导的基因沉默.

主要成果:

确定Ad4bp/sf-1 (类固醇生成因子1) 和Foxp1是与促进体结合的假定转录因子.
Ad4bp/sf-1直接与cyp11b1和cyp17a1促进体结合. Ad4bp/sf-1直接与cyp11b1和cyp17a1促进体结合.
福克斯p1与cyp11b1促进体结合.
在Ad4bp/sf-1表达上,它依赖于雄激素和雄激素.
抑制Ad4bp/sf-1降低了cyp11b1,cyp17a1和Foxp1的表达,并降低了水平.

结论:

Ad4bp/sf-1是丸类固醇发生的关键调节者.
在控制cyp11b1和cyp17a1表达方面,Ad4bp/sf-1和Foxp1起着重要的作用.
淋巴激素和雄激素调节Ad4bp/sf-1表达,突出调节反循环.

关键词:

雄激素依赖性雄激素依赖性促销者动机分析类固醇酶基因是类固醇酶的基因.类固醇原因子的因素这种 siRNA 沉默效应.

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