Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

3.8K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
3.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Alternative lengthening of telomeres and CINSARC are interconnected toward non-translocation-related sarcomas progression.

BMC cancer·2026
Same author

High-grade gliomas and Lynch syndrome: A retrospective descriptive study with a literature review.

Neuro-oncology practice·2026
Same author

Non-coding genome in nail-patella syndrome: Genetic diagnosis as a guide for personalized follow-up.

European journal of human genetics : EJHG·2026
Same author

The neuropsychological outcome of patients undergoing intraoperative MRI surgery for insular infiltrating gliomas.

Journal of neuro-oncology·2026
Same author

Management and survival of patients with cancer of unknown primary discussed by a French national multidisciplinary tumour board: a retrospective analysis.

The Lancet regional health. Europe·2025
Same author

Letter to the editor regarding: "Recurrence patterns and clinical outcomes in adult cerebellar glioblastoma".

Acta neurochirurgica·2025

相关实验视频

Updated: May 5, 2026

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
11:15

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

25.0K

[用于分子生物学分析的哪些样本:先决条件和限制]

Delphine Poncet1, Fabienne Escande2

  • 1Unité de biologie moléculaire somatique, service d'anatomo-cyto-pathologie, groupement hospitalier Est, hospices civils de Lyon, 69394 Bron, France; Team Chromatin Dynamics, Nuclear Domains, Virus, CNRS UMR 5261, Inserm U 1315, LabEx DEVweCAN, Institut NeuroMyoGène (INMG), Pathophysiology and Genetics of the Neuron and Muscle (PGNM) Laboratory, 69008 Lyon, France.

Annales de pathologie
|September 14, 2025
PubMed
概括

适当的样本制备和储存对于准确的分子诊断至关重要. 错误处理生物样本可能导致诊断,预后和预后测试的结果不准确.

关键词:
这就是DNADNADNA.这就是ARN ARN.分子生物学分子生物学质量控制 质量控制它们是DNA DNA DNA DNA.提取 提取 提取分子生物学分子生物学预分析阶段 预分析阶段预分析 (pré-analytique) 是一种预分析方法.质量控制 质量控制这是一个RNARNARNARNARNA.

更多相关视频

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
12:37

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Published on: April 14, 2016

40.1K
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

13.4K

相关实验视频

Last Updated: May 5, 2026

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
11:15

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

25.0K
Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
12:37

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Published on: April 14, 2016

40.1K
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

13.4K

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 癌症研究 癌症研究

背景情况:

  • 细胞和组织样本的分子分析对于诊断,预后和预后至关重要.
  • 生物矩阵 (FFPE,冷组织,液体活检) 的复杂性增加和样本多样性带来了挑战.
  • 瘤细胞含量和核酸质量/数量是分子测试中的关键限制因素.

研究的目的:

  • 突出分子样本处理中的关键预分析步骤.
  • 为了确定从样本错误处理的局限性和潜在的解释偏见.
  • 强调分析前条件对下游分子分析的影响.

主要方法:

  • 对影响分子测试的分析前变量进行审查.
  • 讨论样品制备和储存对核酸完整性的影响.
  • 通过非最佳样本处理引入的潜在偏差的分析.

主要成果:

  • 预分析因素显著影响提取的核酸的质量和数量.
  • 样本完整性与诊断,预后和预后结果的可靠性直接相关.
  • 不一致的样本处理可能导致解释偏见并影响临床决策.

结论:

  • 了解和管理预分析步骤对于可靠的分子诊断至关重要.
  • 适当的样本制备和储存对于确保提取的核酸质量至关重要.
  • 坚持标准化预分析协议将分子测试中的局限性和解释偏见降至最低.