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Updated: Jan 17, 2026

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快速PCR的热循环方法

Ruihua Ding1, Jiali Zhang2, Chang Chen1,2,3

  • 1Shanghai Industrial μTechnology Research Institute (SITRI), Shanghai, China.

Critical reviews in biotechnology
|September 15, 2025
PubMed
概括

快速聚合酶连锁反应 (PCR) 方法通过增强加热/冷却或实现更快的温度区域过渡来加速DNA放大. 这些进展旨在克服传统PCR的时间限制,以更快地检测核酸.

关键词:
这是一个PCRPCR.POCT POCT 在线观看微流体学 在微流体学方面核酸检测检测核酸检测快速加热 快速加热热循环的热循环.

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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