相关实验视频

Updated: Jun 29, 2026

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy

Published on: January 18, 2018

艾滋病毒-1 Tat对FDFT1抑制,胆固醇水平变化和BCBL1细胞中的KSHV复制的影响

Qiaozhen Liu¹, Xiaoying Chen², Dewen Liu³

¹Department of Pathogenic Biology and Immunology, School of Basic Medical Sciences, Youjiang Medical University for Nationalities, Baise, Guangxi, 533000, China.

Current HIV research

|September 15, 2025

概括

人类免疫缺陷病毒1 (HIV-1) Tat蛋白降低法内赛二酸法内赛转移酶1 (FDFT1),改变胆固醇水平并影响卡波西肉瘤相关疹病毒 (KSHV) 复制. 这种分子相互作用影响KSHVV.

科学领域:

病毒学病毒学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

研究了人类免疫缺陷病毒1 (HIV-1) 转录 (Tat) 蛋白的交互激活器在激活卡波西肉瘤相关疹病毒 (KSHV) 复制中的分子机制.
专注于BCBL-1细胞内的HIV-1 Tat和KSHV之间的相互作用.

研究的目的:

为了阐明HIV-1 Tat如何影响KSHV复制.
确定Farnesyl二酸盐Farnesyl转移酶1 (FDFT1) 在这种相互作用中的作用.
为了检查对细胞胆固醇水平的影响.

主要方法:

使用lentivirus在BCBL-1细胞中过度表达HIV-1 Tat.
使用RT-qPCR对FDFT1,HIV-1 Tat,KSHV ORF73和KSHV ORF50进行量化mRNA表达.
评估细胞胆固醇水平和操纵FDFT1表达 (过度表达和RNAi敲击).

主要成果:

在HIV-1 Tat下调的FDFT1和上调的KSHV ORF50表达.
FDFT1过度表达增加了KSHV ORF73 (潜伏相关基因).
FDFT1击败促进了KSHV的性活性化基因.
无论是TAT表达还是FDFT1操纵都显著改变了细胞胆固醇含量.

结论:

艾滋病毒-1 Tat介导的FDFT1下调调节细胞胆固醇.
这种调制与BCBL-1细胞中的KSHV复制动力学密切相关.

关键词:

FDFT1 在线阅读艾滋病病毒-1 艾滋病病毒-1 在 KSHVV 找 KSHV 细胞中的胆固醇含量.复制周期的复制周期这种蛋白质就是这种蛋白质.

更多相关视频

High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing

High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing

Published on: January 22, 2019

Quantitative Fluorescence In Situ Hybridization FISH and Immunofluorescence IF of Specific Gene Products in KSHV-Infected Cells

Quantitative Fluorescence In Situ Hybridization FISH and Immunofluorescence IF of Specific Gene Products in KSHV-Infected Cells

Published on: August 27, 2019

相关概念视频

Herpes

Herpes

Herpes simplex type 1 (HSV‑1) is a widespread pathogen responsible for orolabial lesions. It is an enveloped, double-stranded DNA (dsDNA) virus belonging to the family Herpesviridae. Once the virus infects a host cell, its double‑stranded DNA genome is delivered into the nucleus, where a coordinated cascade of immediate‑early, early, and late gene expression directs viral DNA replication, structural protein synthesis, and virion assembly. After primary infection of epithelial cells, HSV-1...

Hepatitis

Hepatitis

Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver. The...

Antiviral Nucleoside Inhibitors

Antiviral Nucleoside Inhibitors

Antiviral Nucleoside InhibitorsAntiviral nucleoside inhibitors are structural analogs of natural nucleosides that interfere with viral DNA or RNA synthesis. These compounds selectively target viral polymerases due to their resemblance to host nucleosides, thereby disrupting viral genome replication.Mechanism of Acyclovir ActionAcyclovir is a guanosine analog with a three-carbon acyclic side chain. It selectively targets herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2),...