相关实验视频

Updated: Jan 17, 2026

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Published on: May 6, 2022

吸烟相关的DNA甲基化和后代下经验:GUSTO研究的发现

A A Akinkugbe^1,2, C-Y S Hsu³, C Lesseur^1,2

¹Department of Environmental Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

Journal of dental research

|September 15, 2025

概括

产前吸烟暴露,通过DNA甲基化风险得分或自我报告来衡量,与后代牙损伤的增加有关. 这强调了在怀孕期间需要加强戒烟支持,以改善儿童的口腔健康结果.

科学领域:

环境流行病学环境流行病学
遗传学和表观遗传学
儿科牙科儿科牙科

背景情况:

怀孕期间自我报告的吸烟容易导致测量错误,可能会导致与后代脏病的相关性.
之前确定的一种与产前吸烟相关的DNA甲基化 (DNAm) 签名需要进一步验证其与后代虫的关联.
怀孕期吸烟暴露可以通过自我报告和血胆胺被索引,提供替代措施来评估其对儿童健康的影响.

研究的目的:

为了评估产前吸烟相关的DNAm签名和后代牙经验之间的关联.
评估怀孕期吸烟是否与后代脏病有关,并根据自我报告和血中胆固醇含量进行索引.
调查加权DNAm风险评分 (wMRS_DNAm) 与儿童牙表面腐烂数量之间的关系.

主要方法:

利用了新加坡成长向健康结果 (GUSTO) 队列 (n=577个母婴二合体).
通过调查问卷和血中胆固醇,带组织的DNAm和3岁儿童牙损伤评估了孕期吸烟.
采用零膨胀的波桑回归来分析wMRS_DNAm和空经验之间的关联,调整为共变量.

主要成果:

每个wMRS_DNAm的额外四分位数区间 (IQR) 与20%更高的调整后虫风险有关 (RR=1.20,95% CI [1.10,1.31]).
怀孕期间吸烟的母亲 (自我报告/cotinine) 的孩子具有更高的虫风险 (RR=1.31, 95% CI [1.11, 1.55] 轻度吸烟).
从带DNA中获得的DNAm风险评分显示,与更多的后代空经验有积极的关联.

结论:

这些发现证实了产前吸烟暴露与后代脏病增加之间的联系.
母亲吸烟相关的DNAm风险得分与后代更大的空经历有关.
这些结果强调了怀孕期间戒烟支持对于改善儿童口腔健康的重要性.

关键词:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.牙腐烂是指牙的腐烂.甲基化风险评分甲基化风险评分在产前吸烟产前吸烟零膨胀模型的零膨胀模型

更多相关视频

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

相关概念视频

Genomic Imprinting and Inheritance

Genomic Imprinting and Inheritance

Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.