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Ashraf Elsaid¹, Ahmed Fathy State¹, Adel Zalata²

¹Andrology Unit, Department of Dermatology, Andrology & STDs, Faculty of Medicine, Mansoura University, Mansoura, Egypt.

|September 15, 2025

概括

男性的不孕症与较低的miR-339-3p和较高的μ-阿片类受体 (OPRM1) 水平有关,影响精子质量. 这些标记物对诊断男性不孕症有前途.

科学领域:

生殖生物学生殖生物学
分子内分泌学分子内分泌学
安德罗学与人类学

背景情况:

男性不孕症影响了寻求怀孕的大量夫妇.
μ-阿片类受体 (OPRM1) 和与之相关的微RNA,miR-339-3p,与生殖功能有关,但它们在男性不孕症中的作用尚不清楚.

研究的目的:

为了研究 miR-339-3p,OPRM1 mRNA 和 μ-opioid受体蛋白在不孕男性和生育男性的精子中的表达.
评估这些分子标记物与精液参数,氧化应激和荷尔蒙特征之间的关联.

主要方法:

一项以病例为对照的研究,涉及45名不育男性和45名肥育男性.
通过qRT-PCR对miR-339-3p和OPRM1mRNA进行量化.
使用ELISA测量μ-阿片类受体蛋白水平.
综合精液分析,氧化应激标志物评估和血清激素水平评估.

主要成果:

不育的男性显示显著更高的μ-阿片类受体蛋白和OPRM1mRNA水平,显著增加了4.08倍的OPRM1mRNA.
相反,miR-339-3p的表达显著降低 (大约为3. 在不育的男性中0.53倍).
在miR-339-3p和OPRM1mRNA水平之间发现了强烈的负相关性 (r=-0.704).
接收器运行特征分析表明,OPRM1mRNA (AUC=0.916) 和miR-339-3p (AUC=0.914) 的诊断准确度在分辨不育和肥沃个体方面都很好.

结论:

降低miR-339-3p的调节和μ-阿片类受体的过度表达与不孕男性的精液质量受损和氧化失衡有关.
这些分子标记物,miR-339-3p和OPRM1,显示出男性不孕症诊断指标的潜力.

关键词:

在 MiR‐339‐3p 中.OPRM1 的mRNA表达方式.男人的不孕不育症.

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