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相关概念视频

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.8K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

4.7K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
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相关实验视频

Updated: Jan 17, 2026

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
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3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

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动态实验室X射线相位对比微图学与基于结构的先前调整.

Harry Allan1,2, Tom Partridge1, Joseph Jacob3,4

  • 1Department of Medical Physics and Biomedical Engineering, University College London, London, United Kingdom.

Measurement science & technology
|September 15, 2025
PubMed
概括
此摘要是机器生成的。

我们开发了一种更快的X射线微断层图像技术,使用相对比成像和代重建. 这种方法实现了高对比度和时间分辨率,使木结构等样品的动态4D成像成为可能.

关键词:
在4D成像方面,使用4D成像.代的重建重建的重建断层扫描 (tomography) 是一个非常重要的技术.在X射线相位对比的X射线中,相位对比是相位对比.

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Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
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Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

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Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
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Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

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相关实验视频

Last Updated: Jan 17, 2026

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
07:01

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

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Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

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Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
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Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

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科学领域:

  • 材料科学 材料科学 材料科学
  • 图像技术技术的成像技术
  • 物理 物理学 物理

背景情况:

  • 在光学厚度样本的3D结构分析中,X射线微观断层学至关重要.
  • 它的适应4D成像允许在外部刺激下监控动态过程.
  • 实验室来源的X射线流量有限,这对高分辨率动态成像构成了挑战.

研究的目的:

  • 为了提高基于实验室的X射线显微断层扫描中的对比度和时间分辨率.
  • 为了实现动态系统的非破坏性,时间分辨率的3D成像.
  • 为了克服动态样本的传统X射线成像的局限性.

主要方法:

  • 利用自由空间传播相位对比成像进行对比增强.
  • 从参考扫描中实现了以结构为基础的先验的代重建.
  • 通过组合技术实现了高对比度与噪声比率 (CNR) 的改进.

主要成果:

  • 阶段对比成像增加了5.8倍的CNR.
  • 结合方法比传统方法提高了CNR的29.2倍.
  • 启用动态X射线微断层扫描,具有9秒的时间分辨率, voxel大小为10.5μm.

结论:

  • 开发的方法显著提高了动态X射线微断层图像的能力.
  • 通过在木制刺刀中对海运动的成像来证明该技术的有效性.
  • 这种进步为研究各种材料中的快速过程开辟了新的可能性.