通过克服关键的酶瓶来增强 Bacillus subtilis 中的烯生产
Esha Rehman1,2, Hawaibam Birla Singh1,2, Minh Phuong Nguyen1,2
1Anti-Aging Bio Cell Factory Regional Leading Research Center, Gyeongsang National University, Jinju, Republic of Korea.
Frontiers in bioengineering and biotechnology
|September 15, 2025
概括
通过优化前体路径和选择高效的酶,Bacillus subtilis的代谢工程显著增强了利科的产生. 这项工作确立了B. subtilis作为可持续胡卜素生物合成的强大平台.
科学领域:
- 合成生物学和代谢工程合成生物学和代谢工程
- 微生物生物技术 微生物生物技术
- 生物化学和酶工程 生物化学和酶工程
背景情况:
- 细菌细菌是一种安全的GRAS微生物,具有生产高价值化合物的吸引力.
- 之前,B. subtilis的烯生产受到了不高效的前体供应和酶问题的限制.
- 百合素是一种有价值的C40类胡卜素,具有潜在的健康益处.
研究的目的:
- 通过代谢工程来增强Bacillus subtilis中烯的产量.
- 识别和设计关键酶和通路,以实现高效的利科生物合成.
- 开发B. subtilis作为一种可持续的微生物平台,用于生产胡卜素.
主要方法:
- 在B. subtilis.中重新连接烯和甲基利酸 (MEP) 途径.
- 将原生crtE基因用来自Archaeoglobus fulgidus的geranyl二酸盐合成酶 (GGPPS) 替换.
- 优化发酵介质的优化,使用结合的葡萄糖和甘油碳来源.
- 在MEP途径中过度表达1 - 脱氧 - D - 硫 - 5 - 酸盐合成酶 (dxs).
- 选和选择各种GGPPS酶,包括来自Corynebacterium glutamicum的idsA.
主要成果:
- 使用异质GGPPS的合成甘路径使B. subtilis. 能够合成甘.
- 结合的葡萄糖和甘油显著增强了细胞生长和烯生产.
- 过度表达dxs导致白甘标位增加了五倍.
- 这种工程菌株在摇瓶种植中实现了55 mg/L的最终烯标位.
- 使用来自C. glutamicum的idsA进一步改善了柳科的产量.
结论:
- 有针对性的GGPPS选择对于成功的微生物烯生产至关重要.
- 设计MEP路径,特别是dxs,可以增加前体供应和烯产量.
- 通过战略代谢工程,B. subtilis可以被开发成一个强大的,可持续的碳化合物生产平台.
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