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Updated: Jan 17, 2026

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow

Published on: April 18, 2019

在模型细菌膜中对 perfluoroalkyl 物质的膜修饰作用

Micaela Panella¹, Amani Rabadi¹, Jasmin Ceja-Vega¹

¹Department of Chemistry and Biochemistry, Iona University, 715 North Avenue, New Rochelle, New York 10801, United States.

|September 15, 2025

概括

和多基物质 (PFAS) 通过增加透性和破坏脂质包装来破坏细菌膜. 具有较高结合能力的膜,如含有DOPE的膜,更容易受到PFAS污染的影响.

科学领域:

环境化学环境化学
生物化学生物化学
材料科学材料科学材料科学

背景情况:

和多醇基物质 (PFAS) 是广泛存在的环境污染物,具有重大的生态和健康影响.
了解PFAS与生物膜,特别是细菌膜的相互作用,对于评估其影响至关重要.
细菌膜表现出多样化的脂质组成和结构,影响它们对异生物化合物的敏感性.

研究的目的:

研究 perfluorooctanoic 酸 (PFOA) 和 perfluorobutanesulfonic 酸 (PFBS) 对模型细菌膜的影响.
阐明脂质头组类型和负荷在介导PFAS与膜相互作用中的作用.
为了确定PFAS如何影响膜透性,脂质包装和相位行为.

主要方法:

使用各种格式的二元脂质混合物 (DOPC/DOPG和DOPE/DOPG) 构建模型膜 (双层,脂质体,支持双层).
模型膜暴露于不同度的PFOA和PFBS盐.
使用水透性测量,差分扫描热度计 (DSC),拉曼光谱和减弱总反射率红外光谱 (ATR-IR) 评估膜性质.

主要成果:

在DOPC/DOPG和DOPE/DOPG膜中,PFAS盐诱导了度和脂质依赖的破坏性作用.
具有更高结合能力和曲率应力 (含有DOPE) 的膜在暴露于PFAS时显示出更显著的水透性增加.
与DOPC-DOPG混合物相比,DSC,拉曼和ATR-IR光谱检测显示DPE-DOPG混合物中暴露于PFAS的脂酸乙烯链乱程度较大,相位过渡温度降低.

结论:

PFAS盐增加了细菌膜的透性,并破坏了脂质包装和阶段组织的稳定性.
含有DOPE的膜更容易受到PFAS诱导的破坏,因为它们具有更高的结合能力和曲率应力.
该研究强调了静电,范德瓦尔斯和结相互作用的复杂相互作用,这些相互作用控制了PFAS对细菌膜的影响.

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