压力激活神经氧化合成酶的压力激活
Lalita Shahu1, Yadav Prasad Gyawali2, Ting Jiang2
1Center for Photochemical Sciences, Department of Chemistry, Bowling Green State University, Bowling Green, Ohio 43403, United States.
ACS omega
|September 15, 2025
概括
外界力量可以激活通过神经元氧化合成酶 (nNOS) 通过使用无calmodulin (apo-CaM) 作为机械传感器激活氧化 (NO) 生产. 这项研究证明了通过apo-CaM-nNOS相互作用的强力诱导的NO释放.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 卡尔莫杜林 (CaM) 是一种结合的蛋白质,对于通过氧化合成酶 (NOS) 调节氧化 (NO) 生产至关重要.
- 虽然结合的CaM激活了NOS,但无的CaM (apo-CaM) 在NO生物合成中的作用,特别是在机械应力下,仍然未被充分探索.
- 是一种重要的细胞间信号分子,参与许多生理过程.
研究的目的:
- 通过在外部机械力下由apo-CaM激活的神经元NOS (nNOS) 产生NO的机制.
- 为了证明apo-CaM可以作为一个机械传感器来调节nNOS活动.
主要方法:
- 使用原子力显微镜 (AFM) 相关的共聚焦显微镜来探测NO的产生.
- 使用压力来操纵apo-CaM. 使用压力来操纵apo-CaM.
- 使用DAR-4M作为光探针用于NO检测.
主要成果:
- 压力应用于apo-CaM诱导的形状变化.
- 这些强力诱导的apocam变化促进了nNOS酶的结合和激活.
- 通过nNOS在外力下被apo-CaM激活的证明NO生产.
结论:
- Apo-CaM可以作为一种机械感知蛋白,将机械力转化为生物化学信号.
- 外部力量可以通过apo-CaM-nNOS路径直接激活NO生产.
- 这一发现为NO信号的机械调节提供了新的见解.
相关概念视频
Nitric Oxide Signaling Pathway
6.2K
Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...
6.2K
Activation and Inactivation of G Proteins
11.1K
Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
11.1K
Ligand-Gated Ion Channel Receptor: Gating Mechanism
3.8K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
3.8K
Sympathetic Activation
6.6K
The sympathetic division can influence tissues and organs by releasing norepinephrine at peripheral synapses and distributing epinephrine and norepinephrine through the bloodstream. In times of crisis or stress, sympathetic activation occurs, which is regulated by sympathetic centers in the hypothalamus. As a result, sympathetic activation prepares the body for physical exertion, rapid ATP production, and heightened alertness, allowing individuals to respond effectively to challenging or...
6.6K
Smooth Muscle Contraction
7.3K
Smooth muscle contraction is a complex process vital for various bodily functions, from maintaining blood vessel tension to facilitating the movement of food through the digestive tract. Unlike striated muscles, smooth muscle contraction begins more slowly and lasts longer.
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
7.3K
Motor Unit Stimulation
3.5K
When the neuron of a motor unit fires an action potential, it triggers a series of events, leading to a twitch contraction in the muscle fibers. The process of excitation-contraction coupling is crucial in relaying the action potential to the muscle fibers.
The latent period of contraction marks the onset of excitation-contraction coupling, when the action potential propagates across the sarcolemma, preparing the muscle fibers for contraction. As the fibers enter the contraction phase, the...
The latent period of contraction marks the onset of excitation-contraction coupling, when the action potential propagates across the sarcolemma, preparing the muscle fibers for contraction. As the fibers enter the contraction phase, the...
3.5K


