在牛-人界面上对肠毒性甲素耐药黄金葡萄球菌的流行率和抗生素图谱分析
1Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Iranian journal of veterinary research
|September 15, 2025
概括
肠毒性甲素耐药黄金葡萄球菌 (MRSA) 在巴基斯坦的牛和乳制品工人中很常见. 这些抗生素耐药菌株在动物-人类界面构成重大威胁,需要进一步调查.
科学领域:
- 微生物学 微生物学
- 公共卫生 公共卫生
- 兽医科学 兽医科学 兽医科学
背景情况:
- 黄金葡萄球菌是导致牛乳炎和人类食源性疾病的主要原因,原因是肠毒素的产生.
- 甲素耐药黄金葡萄球菌 (MRSA) 是动物和人类的重要病原体.
- 该研究侧重于乳制品动物和相关人类工人的肠毒性MRSA的流行率.
研究的目的:
- 为了确定肠毒性黄金葡萄球菌和MRSA在牛和乳制品工人的流行率.
- 分析这些分离的抗生素耐药性模式.
- 研究动物和人类来源之间的肠毒素基因的遗传关系.
主要方法:
- 聚合酶链反应 (PCR) 用于检测金黄色葡萄球菌,MRSA,spa和肠毒素基因 (seb, sec).
- 分析了来自192头牛和192头水牛的牛奶样本,以及100个人的鼻腔或皮肤拭子.
- 对流行性肠毒素基因序列进行了植物遗传学分析.
主要成果:
- 总体而言,49.79%的分离物是金黄色葡萄球菌,其中29.46%被确定为MRSA.
- 在16.18%的牛和人类分离物中发现了肠毒性黄金葡萄球菌.
- 肠毒性菌株对常见的抗生素表现出耐药性,MRSA患病率在两种人群中均显著.
结论:
- 肠毒性MRSA在巴基斯坦研究地区的牛和奶制品工人中普遍存在.
- 这些分离物表现出多种抗生素耐药性,突出显示了潜在的公共卫生风险.
- 在MRSA中存在毒性因子和抗生素耐药性的存在,强调了它在动物-人类界面的威胁.
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