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Updated: Jan 17, 2026

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
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Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

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在CRISPR-Cas9中扩大PAM兼容性的设计规则从VQR,VRER和EQR变体中扩展

Francisco Vieyra1, Chinmai Pindi2, George P Lisi3,4

  • 1Department of Inorganic, Analytical & Chemical Physics, University of Buenos Aires, Argentina.

bioRxiv : the preprint server for biology
|September 15, 2025
PubMed
概括

扩大CRISPR-Cas9基因组编辑需要识别新的原生空间器相邻基因 (PAM). 这项研究揭示了PAM识别涉及局部DNA稳定和远程网络通信,而不仅仅是直接接触,指导未来的Cas9工程.

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