水产养殖设施特定的微生物群塑造了斑马鱼肠道微生物群
Kayla C Evens1,2, Ingrid Bakke3, Brendan J M Bohannan1
1Department of Biology, Institute of Ecology and Evolution, University of Oregon, Eugene, Oregon, USA.
bioRxiv : the preprint server for biology
|September 15, 2025
概括
斑马鱼 (Danio rerio) 水产养殖系统中的环境微生物群因设施和地理位置而异. 水可以影响鱼的肠道微生物,强调需要进行环境评估,以确保可复制的微生物组研究.
科学领域:
- 微生物学 微生物学
- 水产养殖科学 水产养殖科学
- 主体与微生物的相互作用
背景情况:
- 循环水产养殖系统 (RAS) 中的环境微生物群对与宿主相关的微生物群落产生影响.
- 斑马鱼 (Danio rerio) 的研究可能会经历令人困惑的实验结果,这是由于各种设施之间与宿主相关的微生物群落的不受控制的变化.
- 有限的研究在多个独立设施中描述了水和鱼的微生物群,以评估环境微生物群变异对宿主微生物群的影响.
研究的目的:
- 在美国和挪威的五个水产养殖设施中比较水和斑马鱼肠道微生物组.
- 评估环境微生物群变异对斑马鱼宿主相关微生物群落的影响.
- 评估环境微生物组变异对斑马鱼微生物组研究可重复性的影响.
主要方法:
- 在五个水产养殖设施 (两个美国,三个挪威) 采用了嵌套采样设计.
- 采用16S rRNA基因测序用于对水和斑马鱼肠道样本的微生物社区分析.
- 微生物源追踪的应用相似度百分比分析和快速预期最大化,以确定微生物驱动因素和来源.
主要成果:
- 水中的α多样性高于鱼的肠道;β多样性按样本类型,设施和位置聚合.
- 在各个设施中观察到微生物群落组成的显著差异,在水和鱼类中都有不同的设施特征.
- 坦克水微生物群对斑马鱼肠道社区有可衡量的贡献,微生物相似性随着地理和设施距离的减少而减少.
结论:
- 斑马鱼水产养殖系统拥有独特的微生物群落,受到环境和地理因素的影响.
- 坦克水作为斑马鱼肠道相关微生物的潜在来源,影响宿主微生物组合.
- 纳入环境微生物组评估对于斑马鱼实验设计至关重要,以提高宿主微生物相互作用研究中的可复制性和解释性.
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