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相关概念视频

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

4.5K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.3K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.3K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.3K
Constitutive and Regulated Gene Expression01:27

Constitutive and Regulated Gene Expression

944
Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
944
Real Time RT-PCR02:57

Real Time RT-PCR

64.7K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
64.7K

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相关实验视频

Updated: Jan 17, 2026

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
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Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

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一个节奏基因表达的调度器.

Dimos Gaidatzis1,2, Maike Graf-Landua1,3, Stephen P Methot1

  • 1Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland.

bioRxiv : the preprint server for biology
|September 15, 2025
PubMed
概括
此摘要是机器生成的。

遗传振荡器精确时间基因表达. 这项研究揭示了转录因子结合如何安排C. elegans中的数千个基因,为发育生物学提供了一个框架.

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Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
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相关实验视频

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Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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科学领域:

  • 发展生物学 发展生物学
  • 遗传学 是一个遗传学.
  • 分子生物学分子生物学

背景情况:

  • 遗传振荡器对于在发育和生理学过程中精确的基因表达时间至关重要.
  • C. elegans 变时钟可以作为一个模型系统来理解振荡器如何调节基因表达.
  • 了解定时基因表达的调节机制对于各种生物过程至关重要.

研究的目的:

  • 为了研究基因振荡器如何安排成千上万个基因的表达.
  • 确定控制节律性基因表达的关键调节者和机制.
  • 开发染色体和基因表达动态的预测模型.

主要方法:

  • 单细胞RNA测序用于分析基因表达模式.
  • 时间解析的ATAC-seq用于映射染色体可访问性动态.
  • 开发一个整合转录因子结合数据的线性模型.
  • 扰动实验 (GRH-1/Grainyhead) 用于验证模型预测.

主要成果:

  • 在个体组织中观察到广泛的峰值相分散.
  • 在成千上万的调节元素中,染色质可访问性的节奏变化.
  • 确定了九个关键的转录因子,调节了峰值阶段和振幅.
  • 证明这些因素可以通过破坏性干扰产生非节律活动.
  • 在GRH-1扰动时精确预测基因表达和染色质动态.

结论:

  • 组合性,非合作性转录因子结合提供了一个安排复杂基因表达模式的框架.
  • 确定的监管原则适用于超出发展的动态生物过程.
  • 这项研究提供了对转录因子对基因表达的定量控制的见解.