在Streptococcus sanguinis SK36的自然能力期间,LytF有助于柱状挤出
Rebekka Moe1, Katarzyna Wiaroslawa Piechowiak1, Leiv Sigve Håvarstein1
1Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norway.
血流菌 (LytF) 不会导致兄弟杀戮. 相反,这种能力诱导的酶通过通过增加细胞外柱子成分水平来增强DNA吸收来促进自然转化.
科学领域:
- 微生物学 微生物学
- 细菌遗传学 细菌遗传学
- 分子生物学分子生物学
背景情况:
- 链球菌利用自然转变进行基因交换,需要能力. 一些物种通过细胞壁水解酶 (fratricins) 诱导兄弟杀戮以释放DNA.
- 肺炎 estreptococcus 使用 CbpD 进行兄弟杀戮,但其他链球菌具有能力诱导的 LytF,其功能仍在争论中.
研究的目的:
- 在竞争期间调查LytF在Streptococcus sanguinis中的作用.
- 为了确定LytF是否作为fratricin功能或在自然转化中发挥其他作用.
主要方法:
- 研究了Streptococcus sanguinis中的LytF功能.
- 使用免疫洗和显微镜成像来分析柱子成分水平.
主要成果:
- 没有发现LytF在Streptococcus sanguinis中充当fratricin的作用.
- 通过增强DNA吸收,LytF被证明参与促进自然转化.
- LytF增加了细胞外ComGC水平,这表明它有助于DNA获取的柱状挤出.
结论:
- 在Streptococcus sanguinis中LytF的作用是促进自然转变,而不是兄弟杀戮.
- 通过对二醇糖进行修改,LytF可能通过促进IV型柱体功能来增强DNA吸收.
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