一个杆状cGLR-STING通路揭示了细菌和动物免疫之间的进化联系
Yao Li1,2,3, Hunter C Toyoda1,2, Samantha G Fernandez1,2
1Department of Microbiology, Harvard Medical School, Boston, MA 02115, USA.
bioRxiv : the preprint server for biology
|September 15, 2025
概括
动物的天生的免疫力.
科学领域:
- 进化免疫学的进化免疫学
- 分子生物学分子生物学
- 简介:真核生物的进化过程.
背景情况:
- 动物的天生的免疫源于古老的细菌防御机制.
- 在真核生物中,细菌免疫成分的获取和适应尚未得到充分理解.
- 动物的最亲近的亲属 - - 藻类,为早期的真核免疫提供了洞察力.
研究的目的:
- 为了识别和表征cGLR-STING信号轴在小鞭虫.
- 通过研究细菌到真核生物的基因转移来了解真核生物免疫系统的进化起源.
- 阐明早期免疫信号的分子机制.
主要方法:
- 比较基因组学分析cGLR和STING基因组织.
- 在试验室中复制可阿诺鞭毛体cGLR-STING信号传导.
- 对STING-2'3'-cGAMP复合物的结构分析.
- 在单细胞真核生物中对cGLR和STING同源体的基因分析.
主要成果:
- 在具有混合细菌和动物特征的黄体中确定了一个完整的cGLR-STING信号轴.
- 胆体cGLR和STING基因被组织成类似于操作子的结构,类似于细菌的防御位置.
- 在体外研究证实了通过核酸免疫信号2'3'-cGAMP的激活.
- 结构分析揭示了保留的细菌特征如何影响连接体特异性和受体激活.
- 在其他单细胞真核生物中发现了额外的STING同类物,这表明了多重获取事件.
结论:
- 焦纳鞭状cGLR-STING系统代表着一个分子化石桥梁的细菌和动物免疫力.
- 这项研究揭示了真核细胞免疫系统和基因获取的早期演变.
- 免疫成分的独立获取事件在早期的真核生物进化中多次发生.
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