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相关概念视频

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

12.1K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
12.1K
Heterochromatin02:38

Heterochromatin

17.8K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
17.8K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.3K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.3K
Lampbrush Chromosomes01:51

Lampbrush Chromosomes

8.6K
In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
8.6K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

7.2K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
7.2K
Polytene Chromosomes02:04

Polytene Chromosomes

10.9K
Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also...
10.9K

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Publisher Correction: Interplay between cohesin and RNA polymerase II in regulating chromatin interactions and gene transcription.

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3D chromatin compartment of round spermatids encodes the spatiotemporal program of histone-to-protamine exchange in spermiogenesis.

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HiChIA-Rep quantifies the similarity between enrichment-based chromatin interactions datasets.

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相关实验视频

Updated: Jan 17, 2026

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

18.7K

MIA-Jet:染色体喷流的多尺度识别算法

Sion Kim1,2, Minji Kim1,2

  • 1Gilbert S. Omenn Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, 48109, USA.

bioRxiv : the preprint server for biology
|September 15, 2025
PubMed
概括

研究人员开发了MIA-Jet,这是一种用于检测3D基因组数据中的染色质喷流的新算法. 这种工具揭示了喷射是依赖于凝聚的,并且与DNA复制和基因调节有关,为基因组组织提供了新的见解.

科学领域:

  • 基因组学和分子生物学
  • 计算生物学 计算生物学
  • 3D基因组组织 3D基因组组织

背景情况:

  • 哺乳动物基因组表现出层次组织,包括染色体领土,隔间,域,染色质环和条纹.
  • 染色体喷流是Hi-C地图中最近发现的一种特征,它以垂直于主对角线的分散线出现.
  • 以前的研究表明,染色体喷流可能与凝聚环挤出和DNA复制有关,但由于计算限制,它们的生物学意义仍然不清楚.

研究的目的:

  • 开发一种先进的计算方法,以准确检测具有不同特性的染色体喷流.
  • 通过使用3D基因组映射数据,研究染色体喷射的生物学含义和分子依赖性.
  • 为分析不同基因组数据集和物种的染色体喷流提供一种广泛适用的工具.

主要方法:

  • 开发MIA-Jet,这是一个多尺度峰检测算法,旨在识别染色体喷气.
  • 在各种3D基因组映射数据集上应用和验证MIA-Jet,包括Hi-C,Repli-HiC,ChIA-PET,ChIA-Drop和Micro-C.
  • 在多种物种 (老鼠,人类,圆,斑马鱼) 和实验条件 (蛋白质降解) 中测试MIA-Jet.

主要成果:

  • 与现有方法相比,MIA-Jet在检测各种数据类型和物种的染色体喷流方面表现出更高的性能.
  • 在人体细胞中,检测到的染色质喷射被发现在凝聚素加载部位和早期DNA复制启动区域中被丰富.
关键词:
3D基因组映射 3D基因组映射复制DNA复制DNA复制DNA复制染色体喷流是一种染色体喷流.染色体循环的形成脊柱检测 脊柱检测 脊柱检测 脊柱检测这是一个尺度空间空间.

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Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
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Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

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相关实验视频

Last Updated: Jan 17, 2026

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

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Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
10:41

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

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  • 涉及蛋白质降解的实验表明,染色质喷流依赖于凝聚素,但不是YY1,并且它们的信号在WAPL耗尽时会加剧.

    • 结论:

    • MIA-Jet是一种有效和多功能工具,用于准确检测和分析3D基因组映射数据中的染色质喷流.
    • 染色体喷流在结构上依赖于凝聚体,并在DNA复制和基因组组织等过程中发挥作用.
    • 这些发现为色素喷流的功能作用及其对基因组高阶结构的贡献提供了新的见解.