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CD101在涂抹阴性肺结核的诊断价值

Jingjing Ren^1,2, Shengyan Qu², Keyun Bian³

¹Department of Immunology, School of Medicine, Nantong University, Nantong, China.

Journal of thoracic disease

|September 15, 2025

概括

在CD4+T细胞上的CD101表达有助于诊断样阴性结核病 (SN-TB). 将CD101与IL-2和IFN-γ等细胞因子结合起来,可以提高SN-TB的诊断准确度.

科学领域:

免疫学免疫学免疫学
分子诊断学分子诊断
传染性疾病传染性疾病

背景情况:

涂抹阴性结核病 (SN-TB) 带来了诊断挑战,需要新的分子标记物.
目前对SN-TB的诊断方法不足,导致治疗延迟.

研究的目的:

调查SN-TB患者的外周血液单核细胞 (PBMC),T细胞和NK细胞中的CD101表达.
评估CD101的实用性,单独或与细胞因子一起,用于辅助SN-TB诊断.

主要方法:

病例控制研究比较63名SN-TB患者与59名健康对照 (HC).
在PBMC中检测CD101的RT-qPCR;在T细胞/NK细胞和Th细胞因子上检测CD101的流细胞计.
斯皮尔曼和ROC曲线分析评估了诊断价值.

主要成果:

与HC相比,在SN-TB患者的PBMC和CD4+T细胞中观察到CD101表达的增加.
在SN-TB患者中增加了Th1/Th2细胞因子 (IL-2,IL-4,IL-6,IL-10,TNF-α,IFN-γ),但不是IL-17A.
CD101在CD4+T细胞上与IL-2,IFN-γ,ESR和CRP相关,显示71.43%的灵敏度和71.19%的特异性 (AUC0.7604).
CD101与IL-2和/或IFN-γ的组合改善了诊断性能 (AUC高达0.7899).

结论:

在CD4+ T细胞上的CD101可以区分SN-TB患者与健康个体.
CD101的表达与关键的结核病感染细胞因子和炎症标志物有关.
CD101显示出作为实验室辅助SN-TB诊断的生物标志物的潜力,特别是当与细胞因子结合时.

关键词:

在CD101中,我们可以使用CD101.干扰素-γ (IFN-γ) 的使用.互白素-2 (IL-2) 是一种蛋白质.分子标记物是分子标记物.涂抹阴性肺结核 (SN-TB) 是一种肺结核.

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