CD147通过与HA的直接相互作用,调解了A型流感病毒在细胞表面的吸附
Ting Wang1,2,3, Lei Cao1,2,3, Yufei Zhang1,2
1State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.
Frontiers in cellular and infection microbiology
|September 15, 2025
概括
CD147被确定为一种关键的辅助受体,它与禽流感病毒 (AIV) 血质素 (HA) 结合,促进病毒吸附和复制. 这一发现为开发广泛的抗病毒疗法提供了一个新的目标.
科学领域:
- 病毒学 病毒学
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 禽流感病毒 (AIV) 吸附对其生命周期,宿主热带性和致病性至关重要.
- 虽然已知经典受体,但调解AIV吸附的辅助受体需要进一步阐明.
研究的目的:
- 为了确定与H5N6血凝素 (HA) 相互作用的新型鸟类膜蛋白.
- 研究已识别的蛋白质,特别是CD147在AIV吸附和复制中的作用.
- 为了阐明CD147介导病毒进入的机制.
主要方法:
- 对与H5N6 HA相互作用的鸟类膜蛋白进行查.
- 在DF1和A549细胞中进行过度表达和淘汰实验,以评估CD147的功能.
- 结合性测试以确定CD147和HA之间的直接相互作用.
- 分析CD147糖化及其对病毒吸附的影响.
- 使用可溶性蛋白和单克隆抗体向CD147-HA相互作用的抑制试验.
主要成果:
- 已确定25种与H5N6 HA相互作用的禽类膜蛋白,包括CD147.
- 证实了CD147在促进AIV吸附和复制中的关键作用.
- 证明了CD147的细胞外域与HA受体结合域的直接结合,独立于糖化.
- 表明,艾滋病毒感染高调高糖化CD147 (HG-CD147),而糖化抑制剂降低病毒吸附.
- 抑制CD147-HA相互作用显著降低病毒吸附和复制.
结论:
- CD147作为禽流感病毒 (AIV) 吸附的必要辅助受体.
- CD147和HA之间的相互作用是介导病毒进入的关键机制.
- 准CD147-HA相互作用是开发针对流感病毒的广泛抗病毒疗法的有希望的策略.
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