利用糖醇用于微生物中的二次代谢物生物合成
Kouhei Kamasaka1,2, Luiz Marcello2, Lucília Domingues3,4
1Engineering Biology Research Center, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan.
World journal of microbiology & biotechnology
|September 15, 2025
概括
作为生物柴油副产品的甘油增强了微生物产生的像白醇这样有价值的化合物. 这篇评论探讨了糖醇.
科学领域:
- 生物技术和工业微生物学
- 代谢工程是代谢工程.
- 可持续的生物制造
背景情况:
- 甘油是生物柴油生产的丰富副产品,为其作为微生物原料的使用提供了机会.
- 传统的碳来源,如葡萄糖正在补充或取代糖醇,以增强二次代谢物生产.
- 微生物如Komagataella phaffii和Yarrowia lipolytica有效地利用糖醇进行生物合成.
研究的目的:
- 审查使用甘作为碳源的二次代谢物微生物生产的最新进展.
- 检查参与甘油利用和代谢物生物合成的代谢途径和调节机制.
- 讨论基于糖的生物制造中的潜在应用和挑战.
主要方法:
- 关于草甘醇生物转化最新科学进展的文献综述.
- 对糖同化和二次代谢物合成的代谢途径的分析.
- 检查代谢工程,氧化还原调节和生物过程控制方面的创新.
主要成果:
- 与葡萄糖相比,甘油显著增强了各种二次代谢物的产生,包括芳香化合物,聚醇和脂质.
- 具体的例子显示,使用基于甘油的介质,特别是用氨基酸补充剂,K. phaffii中p-coumarate,naringenin和resveratrol的度增加.
- 最近的创新解决了诸如菌株强度,原始甘油质量和下游加工等挑战.
结论:
- 甘油是一种可持续且具有成本效益的原料,用于微生物生物制造高价值的二次代谢产物.
- 代谢工程和生物过程控制方面的进步正在优化糖的利用.
- 甘油衍生代谢物对制药,食品和生物燃料行业具有重大潜力.
相关概念视频
01:25Lipid Catabolism
862
Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
862
01:24Other Glycolytic Pathways
831
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
831
01:29Biosynthesis of Lipids
532
Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis...
532
01:26Biosynthesis of Polysaccharides
559
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
559
01:20Fates of Pyruvate
10.5K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
10.5K
01:23Glycolysis
1.6K
Glycolysis, the Embden-Meyerhof pathway, is a central metabolic pathway involved in glucose catabolism. It is highly conserved across most organisms, reflecting its fundamental role in cellular energy production. This process occurs in the cytoplasm and can function both in the presence and absence of oxygen, making it versatile for various organisms and environmental conditions.Stages of GlycolysisGlycolysis is a ten-step pathway that converts glucose into pyruvate, generating a net gain of...
1.6K