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Updated: May 2, 2026

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
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混合基基酸盐作为基于灭活动的探头,用于实时成像活性中性粒细胞血清蛋白酶

Jan Pascal Kahler1, Jonathan Coene1, Marcin Skorenski1,2

  • 1Laboratory of Chemical Biology, Department of Cellular and Molecular Medicine, University of Leuven, Herestraat 49, box 901b, 3000 Leuven, Belgium.

ACS chemical biology
|September 16, 2025
PubMed
概括

研究人员开发了新的光探针,以实时检测神经蛋白酶,如中性粒细胞弹性酶. 这些探针可以对活细胞中性粒细胞激活进行成像,从而推进蛋白酶研究.

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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
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