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MALDI-TOF MS 在生物样本中检测氧化主要脂,使用常规矩阵和1-pyrenebutyric Hydrazide

Patricia Prabutzki¹, Jürgen Schiller¹, Kathrin M Engel¹

¹Institute of Medical Physics and Biophysics, Faculty of Medicine, Leipzig University, Härtelstrasse 16-18, 04107 Leipzig, Germany.

Journal of the American Society for Mass Spectrometry

|September 16, 2025

概括

在疾病中分析氧化脂质是具有挑战性的,因为它们的复杂性. 这项研究评估了用于MALDI质谱的矩阵化合物,发现1-pyrenebutyric hydrazide对氧化脂质检测有效.

科学领域:

利皮多米克 (Lipidomics) 是一种消化剂.
生物化学生化学
分析化学分析化学

背景情况:

氧化脂质与与代谢功能障碍和慢性炎症相关的疾病有关.
脂质过氧化产生反应性化物种,这些物种可以破坏DNA和蛋白质等生物分子.
在质谱学中,分析氧化脂质是很困难的,原因是丰度低,结构多样性,短暂性质和矩阵效应.

研究的目的:

通过使用各种矩阵辅助激光脱/电离 (MALDI) 矩阵来评估氧化脂的检测能力.
确定合适的MALDI矩阵,用于分析复杂生物样本中的氧化脂质.
为了评估1-pyrenebutyric化物的实用性,用于MALDI氧化脂质质谱.

主要方法:

评估不同的MALDI矩阵 (2,5-二基酸,4- ((dimethylamino) cinamic酸,9-aminoacridine) 用于氧化脂的检测.
作为复杂的生物标本,对老鼠肝脏提取物的分析.
对1-pyrenebutyric hydrazide作为衍生剂和MALDI矩阵的研究.

主要成果:

不同的MALDI矩阵在检测氧化脂中表现出不同的有效性.
1-pyrenebutyric hydrazide证明了对原生和氧化酸丁胆和酸丁乙醇胺脂的MALDI质谱的适用性.
1-pyrenebutyric hydrazide既可以作为含有的氧化脂质的衍生剂,也可以作为常规的MALDI矩阵.

结论:

选择MALDI矩阵显著影响氧化脂质的检测能力.
1-pyrenebutyric hydrazide为MALDI氧化脂质的质谱分析提供了一种多功能解决方案,包括截断的化物形式.
这一发现有助于在与疾病相关的研究中更好地分析氧化脂质.

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