用Bacillus amyloliquefaciens的多模块化路径工程来加强伊图林A的生产
Zheng-Jie Hou1, Wei Shang1, Kai-Ge Song1
1State Key Laboratory of Synthetic Biology, Frontiers Science Center for Synthetic Biology (Ministry of Education), School of Synthetic Biology and Biomanufacturing, Tianjin University, Yaguan Road 135, Jinnan District, Tianjin 300350, P. R. China.
Journal of agricultural and food chemistry
|September 16, 2025
概括
代谢工程通过优化前体路径,显著提高了Bacillus amyloliquefaciens中的iturin A (一种脂) 生产. 这一突破实现了创纪录的高产量,为工业应用铺平了道路.
科学领域:
- 微生物生物技术 微生物生物技术
- 合成生物学 合成生物学
- 生物化学 生物化学
背景情况:
- 伊图林A是一种有价值的脂,具有多种应用.
- 目前的微生物生产受到前体供应和代谢瓶的限制.
研究的目的:
- 通过模块化代谢工程策略,增强Bacillus amyloliquefaciens中的iturin A生物合成.
- 克服前体可用性和代谢效率低下的局限性.
主要方法:
- 通过修改脂肪酸,林,血清和分支链氨基酸路径来改造Bacillus amyloliquefaciens.
- 操纵的基因参与了前体的合成和降解 (例如,fapR,fabG,mmaA,fadM,sdaAB,proBA,serC,ilvD,gabT).这些基因被操纵了.
- 通过过度表达寡载体 (opp) 运算子来增强的获取.
主要成果:
- 开发了一种工程菌株 (ITUz21),显著改善了伊图林A的生产.
- 获得了7.62g/L的记录iturin A标位.
- 在7.5L生物反应器中达到0.14g/L/h的生产率.
结论:
- 对前体通路的协调工程对于促进微生物iturin A生产是非常有效的.
- 开发的菌株和策略代表了iturin A生物合成的重大进步.
- 这些发现支持大规模工业生产伊图林A.A.的潜力.
相关概念视频
01:22Bioreactor Controls-III
67
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
67
01:27Upstream Processing
97
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
97
01:25Production of Organic Acids
105
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
105
01:27Production of Antibiotics
265
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
265
01:30Production of Pharmaceuticals
94
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
94
01:18Production of Biopesticides
108
Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...
108