素激活的自会在氧化应激下保护BMSC功能:机制和治疗影响
Yurong Chen1,2,3, Qian Peng4, Dongmei Lan1,2,3
1Medical College, Anhui University of Science and Technology, Huainan, China.
Journal of molecular endocrinology
|September 16, 2025
概括
素在氧化应激下增强骨髓 stromal 细胞 (BMSC) 的存活和功能,可能有助于骨质疏松症治疗. 这项研究探讨了素.
科学领域:
- 生物医学科学 生物医学科学
- 细胞生物学 细胞生物学
- 骨质疏松症研究 骨质疏松症研究
背景情况:
- 氧化应激会损害骨髓 stromal 细胞 (BMSCs),这些细胞对骨再生至关重要,也是骨质疏松症的标志.
- 了解保护BMSC免受氧化损伤的因素对于开发骨质疏松症疗法至关重要.
研究的目的:
- 研究素在氧化应激条件下对BMSC增殖和分化的影响.
- 探索素对BMSCs的保护作用的潜在机制.
主要方法:
- 细胞计数套件-8 (CCK-8)用于生命力,反应性氧物种染色,性酸酶 (ALP) 和阿利沙林红色S (ARS) 染色用于骨质生成.
- 西部斑块 (WB) 和实时PCR (RT-PCR) 用于分子分析.
- 免疫组织化学 (IHC) 检测BMSC上的素受体 (TRPV1).
主要成果:
- 当暴露于过氧化 (H2O2) 诱导的氧化应激时,素治疗改善了BMSC活力,抗氧化能力和骨质分化.
- 在BMSC中证实了TRPV1的表达,素增加了细胞内 (Ca2+) 流入和自.
- 素抑制了PI3K/AKT/mTOR信号通路的酸化.
结论:
- 素对BMSC功能有保护作用,可以防止氧化应激.
- 这些保护作用可能是由TRPV1-依赖的Ca2+流入和PI3K/AKT/mTOR通路调节的自作用.
- 素显示出作为治疗骨质疏松症的治疗剂的前景,通过保留BMSC功能.
相关概念视频
Autophagy
5.6K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
5.6K
The Intrinsic Apoptotic Pathway
8.3K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
8.3K
Autophagic Cell Death
4.3K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
4.3K
Delivery Pathways to the Lysosome
8.8K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
8.8K
MAPK Signaling Cascades
7.9K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.9K


