控制pH值的细胞外囊泡从水凝支架中释放,用于治疗应用
Simon Chewchuk1, Nicholas Soucy2, Fan Wan1
1Department of Physics, University of Ottawa, Ottawa, Ontario, K1N 6N5, Canada.
Biomedical materials (Bristol, England)
|September 16, 2025
概括
细胞外囊泡 (EVs) 提供了强大的治疗优势. 这项研究开发了pH响应的水凝微囊,用于针对性地向受损组织输送EV,增强再生疗法并最大限度地减少非目标效应.
科学领域:
- 生物医学工程 生物医学工程
- 再生医学是一种再生医学.
- 纳米技术 纳米技术
背景情况:
- 基于细胞的疗法对组织修复有希望,但存在局限性.
- 细胞外囊泡 (EVs) 正在成为强大的治疗剂,与细胞相比具有优势.
- 针对性地将EVs输送到受损组织仍然是当前治疗策略中的一个重大挑战.
研究的目的:
- 开发一种新的方法,以有针对性地将细胞外囊泡 (EV) 输送到受损组织中.
- 为了创建响应pH的水凝微囊,以控制EV释放.
- 通过确保局部交付来提高基于EV的再生疗法的疗效.
主要方法:
- 在水凝微囊中封装EV的微流体封装.
- 将CD9结合 (CD9BP) 纳入水凝基质中.
- 使用pH敏感的水凝在酸性环境中触发EV释放.
- 在CD9BP功能化的水凝囊中封装CD9+ EVs.
主要成果:
- 在水凝微囊中成功封装了EV.
- 证实在酸性pH的反应中引发了EV内容的释放,模仿受损组织环境.
- 通过响应pH的微囊展示了EV的定向和局部传递.
- 验证了在减少全身暴露的情况下增强组织再生的潜力.
结论:
- 在pH响应的水凝微囊中进行微流体封装,为有针对性的EV输送提供了一个可行的策略.
- 这种方法为局部再生医学应用提供了一个有希望的解决方案.
- CD9BP功能化的水凝系统增强了治疗效果,并最大限度地减少了EVs的非目标效应.
相关概念视频
pH
The potential of hydrogen (pH) is a measure of the acidity or basicity of a water-based solution determined by the concentration of hydronium ions (H3O+). In one liter of pure water at neutral pH, there are 1×10−7 moles of hydronium ions. However, the extensive range of hydronium ion concentrations present in water-based solutions makes measuring pH in moles cumbersome. Therefore, a pH scale was developed to convert moles of hydronium ions into the negative logarithm of the hydronium ion...
pH
The potential of hydrogen (pH) is a measure of the acidity or basicity of a water-based solution determined by the concentration of hydronium ions (H3O+). In one liter of pure water at neutral pH, there are 1×10−7 moles of hydronium ions. However, the extensive range of hydronium ion concentrations present in water-based solutions makes measuring pH in moles cumbersome. Therefore, a pH scale was developed to convert moles of hydronium ions into the negative logarithm of the hydronium ion...
Stomach pH Regulation
The human body carefully regulates the internal pH of different organs to maintain homeostasis. For example, while the blood plasma maintains a neutral pH of 7, the stomach lumen has an acidic pH of 1.5 - 3.5. The low pH of stomach lumen helps kill pathogens in the food and break down complex food molecules.
The acid-secreting gastric mucosal epithelial cells (parietal cells) lining the stomach lumen maintain the low pH in the lumen. Numerous ion transporters and channels on these parietal...
The acid-secreting gastric mucosal epithelial cells (parietal cells) lining the stomach lumen maintain the low pH in the lumen. Numerous ion transporters and channels on these parietal...
pH Regulation in Cells
pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
pH Homeostasis
Acid-base homeostasis is essential for maintaining normal physiological activities in humans. The pH of various body fluids is strictly regulated because it is critical for the optimal activity of enzymes involved in metabolic reactions. Enzymes are basically proteins, so, any significant change in pH can affect their structure and activity. In humans, pH is regulated using three primary mechanisms— chemical buffer systems, respiratory regulation, and renal regulation.
Respiratory Regulation of...
Respiratory Regulation of...
Protein Buffers in Blood Plasma and Cells
The human body utilizes protein buffer systems to maintain a stable pH. These systems capitalize on the dual role of amino acids, which can act as acids or bases by accepting or releasing hydrogen ions in response to pH changes. Protein buffer systems are particularly significant in the extracellular fluid (ECF) and intracellular fluid (ICF) of active cells, where structural and functional proteins provide substantial buffering capacity.
Certain amino acids can exist in a zwitterion state at a...
Certain amino acids can exist in a zwitterion state at a...


