通过皮调节,从高强度糖蜜中增强发酵的生产:性能和机制
Shoujun Zhang1, Chengqi Sun1, Guangping Guo1
1Naval Architecture and Shipping College, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Intelligent Equipment for South China Sea Marine Ranching, Guangdong Ocean University, Zhanjiang 524088, China.
Bioresource technology
|September 16, 2025
概括
与碳酸或没有添加剂相比,贝的调节显著增强了从糖蜜中发酵 (H2) 的产生. 这种方法优化了微生物通路,以获得更大的H2产量.
科学领域:
- 生物技术是生物技术.
- 微生物气生产
- 可持续能源 可持续能源
背景情况:
- 糖蜜是糖精炼的副产品,是发酵的高强度基质.
- 高效的 (H2) 生产对于可再生能源战略至关重要.
- 性添加剂可以影响发酵H2产量,但它们的比较有效性需要研究.
研究的目的:
- 为了评估贝 (OS) 调节在增强高强度糖蜜的发酵H生产中的有效性.
- 为了比较OS调节与碳酸 (CC) 调节,没有性添加剂 (NA).
- 阐明潜在的机制,包括微生物通路和关键元素,负责提高H2产量.
主要方法:
- 在三个条件下使用高强度糖蜜的发酵H2生产实验:贝 (OS),碳酸 (CC) 和没有添加剂 (NA).
- 对H2产量的量化 (mL/L).
- 分析了糖的总用量,pH值,度和液态最终产品.
- 代谢途径分析侧重于NADH生产和消耗途径.
- 确定关键的微生物物种和基本的贡献.
主要成果:
- Oyster shell 调理实现了 2841 mL/L 的 H2 产量,略高于碳酸 (2428 mL/L) 和显著高于没有添加剂 (1702 mL/L).
- 与NA相比,OS和CC两种处理方法在总糖利用,pH,性和液态最终产品方面都显示出明显优异的性能 (p < 0.05).
- 代谢分析显示OS和CC治疗中增强的NADH产生和抑制的NADH消耗途径,表明通过NADH氧化增加H2生成.
- 微生物分析表明,Veillonella和Kurthia在OS的基质利用中起着关键作用,并将和铁确定为有益的元素.
结论:
- 皮调节是一种有效的方法,可以增强从高强度糖蜜中发酵的生产.
- 改善的H2产量归因于微生物代谢的优化,特别是NADH氧化途径.
- 贝中的和铁的存在可能通过激活关键酶进一步促进H2的产生.
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