基于网络药理学,分子对接和实验验验证的20S-protopanaxadiol对糖尿病病的潜在点的分析
Chenfei Li1, Chifa Ma1, Ruxuan Zhao1
1Department of Endocrinology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
Renal failure
|September 16, 2025
概括
来自人参叶中的20S-protopanaxadiol (PPD) 可能会防止糖尿病病 (DN). 在小鼠和细胞中,PPD抑制TGF-β1/Smads通路,减少纤维化和延迟DN进展.
科学领域:
- 药理学和传统医学的医学.
- 分子生物学和生物化学 分子生物学和生物化学
- 科和糖尿病研究研究
背景情况:
- 糖尿病病 (DN) 是糖尿病的一种主要并发症,其特征是逐渐进展的纤维化.
- 人参叶是一种具有潜在治疗功能的传统药物.
- 识别有效的化合物及其对DN的机制对于开发新治疗方法至关重要.
研究的目的:
- 调查20S-protopanaxadiol (PPD) 的保护作用,这是人参叶的关键成分,对糖尿病病 (DN) 的保护作用.
- 阐明PPD作用的基本机制,特别是它对TGF-β1/Smads信号通路的影响.
主要方法:
- 网络药理方法使用TCMSP数据库来识别人参叶子对DN的治疗点.
- 构建蛋白质与蛋白质相互作用 (PPI) 网络和丰富分析 (GO,KEGG).
- 通过分子对接验证网络药理学结果,并评估PPD对db/db小鼠和HK-2细胞中的TGF-β1/Smads通路的影响.
主要成果:
- 鉴定了246个针对人参叶子DN的基因,在AGE-RAGE信号传递和MAPK.K等途径中显著丰富.
- 在db/db小鼠中,PPD的使用改善了功能,并降低了关键纤维化标志物 (TGF-β1,α-SMA,p-Smad3,Smad4).
- 在高葡萄糖诱导的HK-2细胞中,PPD降低了TGF-β1,p-Smad3和Smad4的表达,证实了途径抑制.
结论:
- 20S-protopanaxadiol (PPD) 显示出对糖尿病病的保护作用.
- 通过抑制TGF-β1/Smads信号通路,PPD可以缓解纤维化.
- 这些发现表明PPD是延迟DN进展的潜在治疗剂.
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