质体酸盐转运基因的过度表达导致果中增强的安托西亚宁生物合成
Mengxia Zhang1, Jiahao Zhao2, Nan Wang1,2
1Horticulture Section, School of Integrative Plant Science, Cornell University, Ithaca, New York, USA.
Plant biotechnology journal
|September 17, 2025
概括
果果酸度的调节影响了安东素的合成. 过度表达马拉酸转运基因 (cMa1) 通过降低MdMYB73的调节,增强了安托素的产量,这是一个抑制安托素合成的抑制剂.
科学领域:
- 植物分子生物学 植物分子生物学
- 果实生物化学 果实生物化学
- 代谢工程是代谢工程.
背景情况:
- 安托素决定了水果皮的颜色,通过烯胺路径合成.
- 安西安合成和真空酸盐积累 (果酸性) 之间的联系尚不清楚.
- Ma1编码了一种对果果酸度至关重要的质酸盐转运体.
研究的目的:
- 为了研究果果皮中马拉酸盐运输和安东素生物合成之间的关系.
- 阐明MdMYB73在酸生产中的调节作用.
- 了解Ma1的过度表达如何影响安素合成.
主要方法:
- 在"皇家嘉年华"果中cMa1过度表达.
- 抑制RNAi和暂时过度表达MdMYB73.
- 对氨酸生物合成和基因表达的分析 (MdUFGT,MdMYB73,MdMYB1).
- 染色体免疫沉以评估转录因子的结合.
主要成果:
- 过度表达cMa1增强了氨酸生物合成和下调的MdMYB73表达.
- MdMYB73充当了UDP-葡萄糖的转录抑制剂:黄-3-O-葡萄糖转移酶 (UFGT),这是氨酸合成中的关键酶.
- MdMYB73与激活剂MdMYB1在与MdUFGT促进体结合方面进行竞争.
- 抑制MdMYB73增加了安托西亚宁合成,而其过度表达减少了它.
结论:
- MdMYB73通过抑制MdUFGT和与MdMYB1.1.竞争来负面调节氨酸生物合成.
- 由cMa1过度表达引起的MdMYB73的下调释放MdUFGT抑制,允许增强的安托素生产.
- 这项研究揭示了水果酸度调节和果颜料生物合成之间的新联系.
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