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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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相关实验视频

Updated: Jan 17, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
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可NIR激活的,特定序列的金属核酸支架,用于响应式开.

Arpit Sharma1, Man Kshetri1, Deepak Karna1

  • 1Department of Chemistry and Biochemistry, Kent State University, Kent, Ohio, 44242, USA.

Angewandte Chemie (International ed. in English)
|September 17, 2025
PubMed
概括
此摘要是机器生成的。

我们开发了序列响应诊断解锁,这是一种使用近红外光激活分子的新方法,只有当特定的DNA或miRNA序列存在时才能激活分子. 这促进了向治疗和诊断的发展.

关键词:
通过DNA介导的电子转移.分子机器是分子机器.接近红外的近红外线白金 (IV) 复合物

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科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 纳米技术纳米技术

背景情况:

  • 精确的分子激活与分析物特异性和时空控制是诊断和治疗的挑战.
  • 传统的光无老化缺乏组织透和特异性,而分析剂响应平台缺乏外部控制.

研究的目的:

  • 引入序列响应的诊断解锁,将核酸识别与近红外 (NIR) 触发激活相结合.
  • 开发一种用于精密医学和生物传感的多功能光化学工具.

主要方法:

  • 使用点击化学构建了一个Pt(IV) -DNA分子支架,结合了一个Pt(IV) 中的记者,核酸识别域和NIR天线 (IR800).
  • 利用DNA介导的电子转移 (DNA-MET) 在目标杂交后用于Pt(IV) 中心的光还原.
  • 在溶液和活细胞中进行NIR照射后,证明了光记者 (MCA和BDP) 的脱.

主要成果:

  • 通过互补的DNA或miRNA结合触发的序列响应解锁.
  • 通过DNA-MET证明了高效的记者释放和高序列特异性.
  • 在体外和活细胞环境中验证了平台的功能.

结论:

  • Pt(IV) -DNA支架使响应性分子激活具有高序列特异性和时空控制.
  • 这个平台将诊断和分子激活联系在一起,为精准医学,向药物输送和先进的生物传感提供了潜力.